Application
Fig1: Western blot analysis of RAD50 on human RAD50 recombinant protein using anti-RAD50 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RAD50 on HEK293 (1) and RAD50-hIgGFc transfected HEK293 (2) cell lysate using anti-RAD50 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of RAD50 on C6 (1) and HepG2 (2) cell lysate using anti-RAD50 antibody at 1/1,000 dilution.?Application
Fig4:Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-RAD50 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-RAD50 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with RAD50 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RAD50 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA repair protein RAD50 antibody hRAD50 antibody hsRAD50 antibody Mrell antibody NBSLD antibody Rad 50 antibody RAD50 2 antibody rad50 antibody RAD50 homolog (S cerevisiae) antibody RAD50 homolog antibody RAD50 PEN antibody RAD50, S. cerevisiae, homolog of antibody RAD50_HUMAN antibody RAD502 antibody Rad50l antibody Truncated RAD50 protein antibody |
| Product description | Rad52 family members (Rad50, Rad51B/C/D, Rad52, Rad54, MRE11) mediate DNA double-strand break repair (DSBR) for DNA damage that otherwise could cause cell death, mutation or neoplastic transformation. Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands. Rad54A of the DEAD-like helicase superfamily binds to double-stranded DNA and induces a DNA topological change, which is thought to facilitate homologous DNA pairing and stimulate DNA recombination. Rad54B of the DEAD-like helicase superfamily binds to double-stranded DNA and displays ATPase activity in the presence of DNA. Rad54B is abundant in testis and spleen, and mutations of this gene occur in primary lymphoma and colon cancer. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | DNA repair protein RAD50 antibody
hRAD50 antibody
hsRAD50 antibody
Mrell antibody
NBSLD antibody
Rad 50 antibody
RAD50 2 antibody
rad50 antibody
RAD50 homolog (S cerevisiae) antibody
RAD50 homolog antibody
RAD50 PEN antibody
RAD50 S. cerevisiae homolog of antibody RAD50_HUMAN antibody RAD502 antibody Rad50l antibody Truncated RAD50 protein antibody |
|---|---|
| Molecular Weight(MW) | 154 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q92878 |
|---|
Application
Fig1: Western blot analysis of RAD50 on human RAD50 recombinant protein using anti-RAD50 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RAD50 on HEK293 (1) and RAD50-hIgGFc transfected HEK293 (2) cell lysate using anti-RAD50 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of RAD50 on C6 (1) and HepG2 (2) cell lysate using anti-RAD50 antibody at 1/1,000 dilution.?
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-RAD50 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-RAD50 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with RAD50 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RAD50 recombinant protein, RAD50-hIgGFc transfected HEK293 cell lysate, C6, HepG2, Hela, human esophageal cancer tissue, human rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
