Application
Fig1: Western blot analysis of ULK2 on human ULK2 recombinant protein using anti-ULK2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ULK2 on HEK293 (1) and ULK2-hIgGFc transfected HEK293 (2) cell lysate using anti-ULK2 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of ULK2 on different cell lysate using anti-ULK2 antibody at 1/1,000 dilution. Positive control： Lane1: NIH/3T3 Lane2: HepG2 Lane3: SK-Hep-1 Lane4: SK-OV-3 Lane5: C6 Lane6: PC-12 Lane7: MCF-7Application
Fig4:Flow cytometric analysis of Hela cells with ULK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ULK2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG1B antibody KIAA0623 antibody Serine/threonine protein kinase ULK2 antibody Serine/threonine-protein kinase ULK2 antibody ULK2 antibody ULK2_HUMAN antibody Unc 51 (C. elegans) like kinase 2 antibody Unc 51 like autophagy activating kinase 2 antibody Unc 51 like kinase 2 antibody Unc-51-like kinase 2 antibody Unc51.2 antibody |
| Product description | ULK1 and ULK2 (for UNC-51-like kinase) encode similar amino-terminal serine/threonine kinase domains, a proline/serine-rich (PS) domain, and a species-conserved carboxyl-terminal domain. Both share homology with the UNC-51 kinase from Caenorhabditis elegans and the APG1 kinase in yeast, which are involved in axonal extension and growth, and autophagy, respectively. ULK1 maps to human chromosome 12q24.33 and is ubiquitously expressed. ULK2, also widely expressed, maps to mouse chromosome 11 B2 and is expected to have a similar molecular weight as ULK1 in human. ULK1 and ULK2 are thought to autophosphorylate the PS domain in vitro, and the significant homology among vertebrates suggest that ULK1 and ULK2 are involved in the regulation of fundamental biological processes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | ATG1B antibody KIAA0623 antibody Serine/threonine protein kinase ULK2 antibody Serine/threonine-protein kinase ULK2 antibody ULK2 antibody ULK2_HUMAN antibody Unc 51 (C. elegans) like kinase 2 antibody Unc 51 like autophagy activating kinase 2 antibody Unc 51 like kinase 2 antibody Unc-51-like kinase 2 antibody Unc51.2 antibody |
|---|---|
| Molecular Weight(MW) | 113 kDa |
| Cellular Localization | Cytoplasmic vesicle membrane. Peripheral membrane protein. |
Application
Fig1: Western blot analysis of ULK2 on human ULK2 recombinant protein using anti-ULK2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ULK2 on HEK293 (1) and ULK2-hIgGFc transfected HEK293 (2) cell lysate using anti-ULK2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of ULK2 on different cell lysate using anti-ULK2 antibody at 1/1,000 dilution. Positive control： Lane1: NIH/3T3 Lane2: HepG2 Lane3: SK-Hep-1 Lane4: SK-OV-3 Lane5: C6 Lane6: PC-12 Lane7: MCF-7
Application
Fig4:Flow cytometric analysis of Hela cells with ULK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ULK2 recombinant protein, ULK2-hIgGFc transfected HEK293 cell lysate, NIH/3T3, HepG2, SK-Hep-1, SK-OV-3, C6, PC-12, MCF-7, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
