Application
Fig1: Western blot analysis of GFPT1 on human GFPT1 recombinant protein using anti-GFPT1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of GFPT1 on HEK293 (1) and GFPT1-hIgGFc transfected HEK293 (2) cell lysate using anti-GFPT1 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of GFPT1 on different cell lysate using anti-GFPT1 antibody at 1/1,000 dilution. Positive control： Lane1: HepG2 Lane2: HEK293 Lane3: BEL-7402 Lane4: SMMC-7721 Lane5: SK-MES-1 Lane6: C6 Lane7: COS7Application
Fig4:ICC staining GFPT1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Hela cells with GFPT1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | GFPT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMS12 antibody CMSTA1 antibody D-fructose-6-phosphate amidotransferase 1 antibody GFA antibody GFAT 1 antibody GFAT antibody GFAT1 antibody GFAT1m antibody GFPT antibody Gfpt1 antibody GFPT1_HUMAN antibody GFPT1L antibody Glucosaminefructose-6-phosphate aminotransferase [isomerizing] 1 antibody Glutaminefructose-6-phosphate transaminase 1 antibody Glutamine:fructose 6 phosphate amidotransferase 1 antibody Hexosephosphate aminotransferase 1 antibody MSLG antibody |
| Product description | Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1. Defects in GFPT1 are the cause of limb-girdle myasthenia with tubular aggregates (LGMTA). A congenital myasthenic syndrome characterized by onset of proximal muscle weakness in the first decade. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMonkeyRat |
| Concentration | 2mg/ml |
| Alternative Names | CMS12 antibody CMSTA1 antibody D-fructose-6-phosphate amidotransferase 1 antibody GFA antibody GFAT 1 antibody GFAT antibody GFAT1 antibody GFAT1m antibody GFPT antibody Gfpt1 antibody GFPT1_HUMAN antibody GFPT1L antibody Glucosaminefructose-6-phosphate aminotransferase [isomerizing] 1 antibody Glutaminefructose-6-phosphate transaminase 1 antibody Glutamine:fructose 6 phosphate amidotransferase 1 antibody Hexosephosphate aminotransferase 1 antibody MSLG antibody |
|---|---|
| Molecular Weight(MW) | 79 kDa |
| Cellular Localization | Cytosol. Extracellular exosome. |
Application
Fig1: Western blot analysis of GFPT1 on human GFPT1 recombinant protein using anti-GFPT1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of GFPT1 on HEK293 (1) and GFPT1-hIgGFc transfected HEK293 (2) cell lysate using anti-GFPT1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of GFPT1 on different cell lysate using anti-GFPT1 antibody at 1/1,000 dilution. Positive control： Lane1: HepG2 Lane2: HEK293 Lane3: BEL-7402 Lane4: SMMC-7721 Lane5: SK-MES-1 Lane6: C6 Lane7: COS7
Application
Fig4:ICC staining GFPT1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Hela cells with GFPT1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human GFPT1 recombinant protein, GFPT1-hIgGFc transfected HEK293 cell lysate, HepG2, HEK293, BEL-7402, SMMC-7721, SK-MES-1, C6, COS7, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
