Application
Fig1: Western blot analysis of PPARA on human PPARA recombinant protein using anti-PPARA antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PPARA on HEK293 (1) and PPARA-hIgGFc transfected HEK293 (2) cell lysate using anti-PPARA antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of PPARA on different cell lysate using anti-PPARA antibody at 1/1,000 dilution. Positive control： Lane1: ?HepG2 Lane2: HEK293 Lane3: COS7 Lane4: HepG2 Lane5: JurkatApplication
Fig4:Flow cytometric analysis of Jurkat cells with PPARA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PPARA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hPPAR antibody MGC2237 antibody MGC2452 antibody NR1C1 antibody Nuclear receptor subfamily 1 group C member 1 antibody OTTHUMP00000197740 antibody OTTHUMP00000197741 antibody Peroxisome proliferative activated receptor alpha antibody Peroxisome proliferator activated receptor alpha antibody Peroxisome proliferator-activated receptor alpha antibody PPAR antibody PPAR-alpha antibody ppara antibody PPARA_HUMAN antibody PPARalpha antibody |
| Product description | Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that can be activated by a variety of compounds including fibratus, thiazolidinediones, prostaglandins and fatty acids. Three PPAR subtypes, designated PPARα, PPARβ (also designated PPARδ) and PPARγ, have been described. PPARs promote transcription by forming heterodimers with members of the retinoid X receptor (RXR) family of steroid receptors and binding to specific DNA motifs termed PPAR-response elements (PPREs). PPARα is abundant in primary hepatocytes where it regulates the expression of proteins involved in fatty acid metabolism. Interestingly, both the orphan nuclear hormone receptor LXRα and thyroid receptor (TR) have been shown to act as antagonists of PPARα/RXRα binding to PPREs. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 2mg/ml |
| Alternative Names | hPPAR antibody MGC2237 antibody MGC2452 antibody NR1C1 antibody Nuclear receptor subfamily 1 group C member 1 antibody OTTHUMP00000197740 antibody OTTHUMP00000197741 antibody Peroxisome proliferative activated receptor alpha antibody Peroxisome proliferator activated receptor alpha antibody Peroxisome proliferator-activated receptor alpha antibody PPAR antibody PPAR-alpha antibody ppara antibody PPARA_HUMAN antibody PPARalpha antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q07869 |
|---|
Application
Fig1: Western blot analysis of PPARA on human PPARA recombinant protein using anti-PPARA antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PPARA on HEK293 (1) and PPARA-hIgGFc transfected HEK293 (2) cell lysate using anti-PPARA antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PPARA on different cell lysate using anti-PPARA antibody at 1/1,000 dilution. Positive control： Lane1: ?HepG2 Lane2: HEK293 Lane3: COS7 Lane4: HepG2 Lane5: Jurkat
Application
Fig4:Flow cytometric analysis of Jurkat cells with PPARA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PPARA recombinant protein, PPARA-hIgGFc transfected HEK293 cell lysate, HepG2, HEK293, COS7, HepG2, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
