Application
Fig1: Western blot analysis of RND3 on human RND3 recombinant protein using anti-RND3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RND3 on HEK293 (1) and RND3-hIgGFc transfected HEK293 (2) cell lysate using anti-RND3 antibody at 1/1,000 dilution.Application
Fig3: ICC staining RND3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RND3 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with RND3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RND3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARHE antibody memB antibody Protein MemB antibody Ras homolog gene family member E antibody Rho family GTPase 3 antibody Rho-related GTP-binding protein Rho8 antibody Rho-related GTP-binding protein RhoE antibody Rho8 antibody RhoE antibody Rnd3 antibody RND3_HUMAN antibody Small GTP binding protein Rho8 antibody |
| Product description | The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ARHE antibody
memB antibody Protein MemB antibody Ras homolog gene family member E antibody Rho family GTPase 3 antibody Rho-related GTP-binding protein Rho8 antibody Rho-related GTP-binding protein RhoE antibody Rho8 antibody RhoE antibody Rnd3 antibody RND3_HUMAN antibody Small GTP binding protein Rho8 antibody |
|---|---|
| Molecular Weight(MW) | 27 kDa |
| Cellular Localization | Golgi apparatus membrane. |
| SwissProt ID | P61587 |
|---|
Application
Fig1: Western blot analysis of RND3 on human RND3 recombinant protein using anti-RND3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RND3 on HEK293 (1) and RND3-hIgGFc transfected HEK293 (2) cell lysate using anti-RND3 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining RND3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RND3 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with RND3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RND3 recombinant protein, RND3-hIgGFc transfected HEK293 cell lysate, Hela, human bladder cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
