Application
Fig1: Western blot analysis of CHRNA6 on ?human CHRNA6 recombinant protein using anti-CHRNA6 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CHRNA6 on HEK293 (1) and CHRNA6-hIgGFc transfected HEK293 (2) cell lysate using anti-CHRNA6 antibody at 1/1,000 dilution.Application
Fig3:ICC staining CHRNA6 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CHRNA6 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of SH-SY5Y cells with CHRNA6 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).Application
Fig6: Flow cytometric analysis of SK-N-SH cells with CHRNA6 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CHRNA6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AChR-alpha6 antibody Acra6 antibody Cholinergic receptor nicotinic alpha 6 antibody Cholinergic receptor nicotinic alpha polypeptide 6 antibody CHRNA 6 antibody nAChR alpha 6 antibody NACHRA6 antibody Neuronal acetylcholine receptor subunit alpha 6 antibody Nicotinic acetylcholine receptor alpha6 antibody |
| Product description | Nicotinic acetylcholine receptors (nAChRs, AChRs) are protein complexes made up of protein subunits that coassemble to form an ion channel. This ion channel is gated through the binding of the neurotransmitter acetylcholine (ACh) to its ligand-binding site on the AChR. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. CHRNA6 (cholinergic nicotinic receptor alpha polypeptide 6) is a neuronal acetylcholine receptor protein. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:200-1:1,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AChR-alpha6 antibody Acra6 antibody Cholinergic receptor nicotinic alpha 6 antibody Cholinergic receptor nicotinic alpha polypeptide 6 antibody CHRNA 6 antibody nAChR alpha 6 antibody NACHRA6 antibody Neuronal acetylcholine receptor subunit alpha 6 antibody Nicotinic acetylcholine receptor alpha6 antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Cell junction. Cell membrane. Multi-pass membrane protein. |
| SwissProt ID | Q15825 |
|---|
Application
Fig1: Western blot analysis of CHRNA6 on ?human CHRNA6 recombinant protein using anti-CHRNA6 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CHRNA6 on HEK293 (1) and CHRNA6-hIgGFc transfected HEK293 (2) cell lysate using anti-CHRNA6 antibody at 1/1,000 dilution.
Application
Fig3:ICC staining CHRNA6 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CHRNA6 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of SH-SY5Y cells with CHRNA6 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Application
Fig6: Flow cytometric analysis of SK-N-SH cells with CHRNA6 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CHRNA6 recombinant protein, CHRNA6-hIgGFc transfected HEK293 cell lysate, Hela, SK-N-SH, SH-SY5Y, human bladder cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:200-1:1,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
