Application
Fig1: Western blot analysis of CD62L on human CD62L recombinant protein using anti- CD62L antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CD62L on HEK293 (1) and CD62L -hIgGFc transfected HEK293 (2) cell lysate using anti- CD62L antibody at 1/1,000 dilution.Application
Fig3: ICC staining CD62L (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:ICC staining CD62L (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | SELL |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A.11 antibody AI528707 antibody CD62 antigen ligand antibody CD62 antigen-like family member L antibody CD62L antibody gp90-MEL antibody IgA nephropathy, susceptibility to, included antibody L Selectin antibody L-selectin antibody LAM-1 antibody LAM1 antibody LECAM1 antibody LEU8 antibody Leukocyte adhesion molecule 1 antibody Leukocyte surface antigen Leu-8 antibody Leukocyte-endothelial cell adhesion molecule 1 antibody Lnhr antibody LSEL antibody Ly-22 antibody Ly-m22 antibody Lyam-1 antibody LYAM1 antibody LYAM1_HUMAN antibody Lymph node homing receptor antibody Lymphocyte adhesion molecule 1 antibody Lymphocyte antigen 22 antibody Lymphocyte surface MEL-14 antigen antibody MEL-14 antibody Pln homing receptor antibody PLNHR antibody Selectin L antibody Selectin, lymphocyte antibody SELL antibody TQ1 antibody |
| Product description | Selectins, also designated SELL antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Lex as a ligand and bind to specific carbohydrates on neutrophils and monocytes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:500-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | A.11 antibody
AI528707 antibody
CD62 antigen ligand antibody
CD62 antigen-like family member L antibody
CD62L antibody
gp90-MEL antibody
IgA nephropathy susceptibility to included antibody L Selectin antibody L-selectin antibody LAM-1 antibody LAM1 antibody LECAM1 antibody LEU8 antibody Leukocyte adhesion molecule 1 antibody Leukocyte surface antigen Leu-8 antibody Leukocyte-endothelial cell adhesion molecule 1 antibody Lnhr antibody LSEL antibody Ly-22 antibody Ly-m22 antibody Lyam-1 antibody LYAM1 antibody LYAM1_HUMAN antibody Lymph node homing receptor antibody Lymphocyte adhesion molecule 1 antibody Lymphocyte antigen 22 antibody Lymphocyte surface MEL-14 antigen antibody MEL-14 antibody Pln homing receptor antibody PLNHR antibody Selectin L antibody Selectin lymphocyte antibody SELL antibody TQ1 antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P14151 |
|---|
Application
Fig1: Western blot analysis of CD62L on human CD62L recombinant protein using anti- CD62L antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CD62L on HEK293 (1) and CD62L -hIgGFc transfected HEK293 (2) cell lysate using anti- CD62L antibody at 1/1,000 dilution.
Application
Fig3: ICC staining CD62L (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:ICC staining CD62L (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Human CD62L recombinant protein, CD62L -hIgGFc transfected HEK293 cell lysate, Hela, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
