Application
Fig1: Western blot analysis of RAB11FIP1 on human RAB11FIP1 recombinant protein using anti-RAB11FIP1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RAB11FIP1 on HEK293 (1) and RAB11FIP1-hIgGFc transfected HEK293 (2) cell lysate using anti-RAB11FIP1 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of RAB11FIP1 on different cell lysate using anti-RAB11FIP1 antibody at 1/1,000 dilution. Positive control： Lane1: Raji Lane2: SW620 Lane3: A431 Lane4: SW480 Lane5: HepG2 Lane6: Hela Lane7: NIH/3T3Application
Fig4:ICC staining RAB11FIP1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-RAB11FIP1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RAB11FIP1 antibody. Counter stained with hematoxylin.Application
Fig7:Flow cytometric analysis of Raji cells with RAB11FIP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RAB11FIP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ22524 antibody FLJ22622 antibody HGNC:30265 antibody MGC78448 antibody No Rab11 binding protein 1A antibody NOEL1A antibody Rab coupling protein antibody Rab effector protein antibody Rab interacting recycling protein antibody Rab-coupling protein antibody RAB11 family interacting protein 1 (class I) antibody Rab11 family interacting protein 1D antibody Rab11 family-interacting protein 1 antibody Rab11 FIP1 antibody Rab11-FIP1 antibody RAB11FIP1 antibody RCP antibody RFIP1_HUMAN antibody |
| Product description | A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | FLJ22524 antibody FLJ22622 antibody HGNC:30265 antibody MGC78448 antibody No Rab11 binding protein 1A antibody NOEL1A antibody Rab coupling protein antibody Rab effector protein antibody Rab interacting recycling protein antibody Rab-coupling protein antibody RAB11 family interacting protein 1 (class I) antibody Rab11 family interacting protein 1D antibody Rab11 family-interacting protein 1 antibody Rab11 FIP1 antibody Rab11-FIP1 antibody RAB11FIP1 antibody RCP antibody RFIP1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 137 kDa |
| Cellular Localization | Recycling endosome. Cytoplasmic vesicle, phagosome membrane. Cytoplasm. |
Application
Fig1: Western blot analysis of RAB11FIP1 on human RAB11FIP1 recombinant protein using anti-RAB11FIP1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RAB11FIP1 on HEK293 (1) and RAB11FIP1-hIgGFc transfected HEK293 (2) cell lysate using anti-RAB11FIP1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of RAB11FIP1 on different cell lysate using anti-RAB11FIP1 antibody at 1/1,000 dilution. Positive control： Lane1: Raji Lane2: SW620 Lane3: A431 Lane4: SW480 Lane5: HepG2 Lane6: Hela Lane7: NIH/3T3
Application
Fig4:ICC staining RAB11FIP1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-RAB11FIP1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RAB11FIP1 antibody. Counter stained with hematoxylin.
Application
Fig7:Flow cytometric analysis of Raji cells with RAB11FIP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RAB11FIP1 recombinant protein, RAB11FIP1-hIgGFc transfected HEK293 cell lysate, Raji, SW620, A431, SW480, HepG2, Hela, NIH/3T3, human bladder cancer tissue, human cervical cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
