Application
Fig1: Western blot analysis of AOF1 on human AOF1 recombinant protein using anti-AOF1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of AOF1 on HEK293 (1) and AOF1-hIgGFc transfected HEK293 (2) cell lysate using anti-AOF1 antibody at 1/1,000 dilution.Application
Fig3: ICC staining AOF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | AOF1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | amine oxidase (flavin containing) domain 1 antibody amine oxidase, flavin containing 1 antibody AOF1 antibody bA204B7.3 antibody C6orf193 antibody dJ298J15.2 antibody Flavin-containing amine oxidase domain-containing protein 1 antibody FLJ33898 antibody FLJ34109 antibody FLJ43328 antibody KDM1B antibody KDM1B_HUMAN antibody LSD2 antibody lysine (K)-specific demethylase 1B antibody Lysine-specific demethylase 2 antibody Lysine-specific histone demethylase 1B antibody Lysine-specific histone demethylase 2 antibody |
| Product description | AOF1 (amine-oxidase flavin-containing domain 1), whose alternative names include KDM1B (lysine (K)-specific demethylase 1B) or LSD2 (lysine-specific histone demethylase 2), is an 823 amino acid nuclear protein belonging to the flavin monoamine oxidase family. As a histone demethylase, AOF1 specifically demethylates Lys4 of histone H3, a marker for epigenetic transcriptional activation, by interacting with a long stretch of the H3 N-terminal tail of mono- and dimethylated Lys4. Existing as three alternatively spliced isoforms, AOF1 is required for de novo DNA methylation of some imprinted genes in oocytes and contains a single SWIRM domain that is implicated in chromatin regulation. AOF1 contains one CW-type zinc finger and is encoded by a gene located on human chromosome 6p22.3. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | amine oxidase (flavin containing) domain 1 antibody
amine oxidase flavin containing 1 antibody AOF1 antibody bA204B7.3 antibody C6orf193 antibody dJ298J15.2 antibody Flavin-containing amine oxidase domain-containing protein 1 antibody FLJ33898 antibody FLJ34109 antibody FLJ43328 antibody KDM1B antibody KDM1B_HUMAN antibody LSD2 antibody lysine (K)-specific demethylase 1B antibody Lysine-specific demethylase 2 antibody Lysine-specific histone demethylase 1B antibody Lysine-specific histone demethylase 2 antibody |
|---|---|
| Molecular Weight(MW) | 92 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q8NB78 |
|---|
Application
Fig1: Western blot analysis of AOF1 on human AOF1 recombinant protein using anti-AOF1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of AOF1 on HEK293 (1) and AOF1-hIgGFc transfected HEK293 (2) cell lysate using anti-AOF1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining AOF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human AOF1 recombinant protein, AOF1-hIgGFc transfected HEK293 cell lysate, Hela, human esophageal cancer, human rectum cancer. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
