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Fig1: Western blot analysis of IL1RAPL1 on ?human IL1RAPL1 recombinant protein using anti-IL1RAPL1 antibody at 1/1,000 dilution.
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Fig2: Western blot analysis of IL1RAPL1 on HEK293 (1) and IL1RAPL1-hIgGFc transfected HEK293 (2) cell lysate using anti-IL1RAPL1 antibody at 1/1,000 dilution.
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Fig3: Western blot analysis of IL1RAPL1 on different cell lysate using anti-IL1RAPL1 antibody at 1/1,000 dilution. Positive control: Lane1: A431 Lane2: SK-Hep-1 Lane3: HL-7702
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Fig4:ICC staining IL1RAPL1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Fig5: Flow cytometric analysis of Hela cells with IL1RAPL1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).