Application
Fig1: Western blot analysis of ADAR on human ADAR recombinant protein using anti-ADAR antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ADAR on HEK293 (1) and ADAR-hIgGFc transfected HEK293 (2) cell lysate using anti-ADAR antibody at 1/1,000 dilution.Application
Fig3: ICC staining ADAR (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Flow cytometric analysis of Hela cells with ADAR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).Application
Fig5: Flow cytometric analysis of Jurkat cells with ADAR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ADAR [E4-E2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 136 kDa double-stranded RNA-binding protein antibody 136kDa double stranded RNA binding protein antibody Adar 1 antibody ADAR antibody Adar1 antibody Adenosine deaminase acting on RNA 1 A antibody Adenosine deaminase RNA specific 1 antibody Adenosine deaminase RNA specific antibody Adenosine deaminase that act on RNA antibody AGS6 antibody AV242451 antibody Double stranded RNA specific adenosine deaminase antibody Double-stranded RNA-specific adenosine deaminase antibody Double-stranded RNA-specific editase Adar antibody DRADA antibody Dsh antibody Dsrad antibody DSRAD_HUMAN antibody dsRNA adenosine deaminase antibody EC 3.5.4.- antibody G1P1 antibody IFI 4 antibody IFI-4 antibody IFI4 antibody Ifi4 protein antibody Interferon induced protein 4 antibody Interferon inducible protein 4 antibody Interferon-inducible protein 4 antibody K88DSRBP antibody mZaADAR antibody P136 antibody Pre-mRNA adenosine deaminase antibody RNA adenosine deaminase 1 antibody RNA-editing deaminase 1 antibody RNA-editing enzyme 1 antibody |
| Product description | RNA-specific adenosine deaminase (ADAR1, DSH, IFI4, p136, DRADA, DSRAD, K88dsRBP) mediates RNA editing by destabilizing double stranded RNA through deamination of adenosine to inosine in structured or double-stranded RNAs. ADAR1 is expressed from an interferon-response promoter and has a Z-DNA/Z-RNA binding domain at its N-terminus. ADAR1 co-localizes with SUMO-1 in a subnucleolar region that is distinct from the fibrillar center, the dense fibrillar component and the granular component. Localization of nuclear ADAR1 is under the influence of a nucleolar localization signal (NoLS) in the middle of ADAR1 and the exporting activity of the nuclear exporter signal (NES) near the N terminus. ADAR1 upregulates nuclear factor 90 (NF90)-mediated gene expression by interacting with NF110, NF90 and NF45. ADAR1 binds short interfering RNA (siRNA), and gene silencing by siRNA is significantly more effective in mouse fibroblasts homozygous for an ADAR1 null mutation than in wild-type cells. ADAR1 may limit the efficacy of siRNA in mammalian cells. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 136 kDa double-stranded RNA-binding protein antibody 136kDa double stranded RNA binding protein antibody Adar 1 antibody ADAR antibody Adar1 antibody Adenosine deaminase acting on RNA 1 A antibody Adenosine deaminase RNA specific 1 antibody Adenosine deaminase RNA specific antibody Adenosine deaminase that act on RNA antibody AGS6 antibody AV242451 antibody Double stranded RNA specific adenosine deaminase antibody Double-stranded RNA-specific adenosine deaminase antibody Double-stranded RNA-specific editase Adar antibody DRADA antibody Dsh antibody Dsrad antibody DSRAD_HUMAN antibody dsRNA adenosine deaminase antibody EC 3.5.4.- antibody G1P1 antibody IFI 4 antibody IFI-4 antibody IFI4 antibody Ifi4 protein antibody Interferon induced protein 4 antibody Interferon inducible protein 4 antibody Interferon-inducible protein 4 antibody K88DSRBP antibody mZaADAR antibody P136 antibody Pre-mRNA adenosine deaminase antibody RNA adenosine deaminase 1 antibody RNA-editing deaminase 1 antibody RNA-editing enzyme 1 antibody |
|---|---|
| Molecular Weight(MW) | 136 kDa |
| Cellular Localization | Cytoplasm. Nucleus > nucleolus. |
| SwissProt ID | P55265 |
|---|
Application
Fig1: Western blot analysis of ADAR on human ADAR recombinant protein using anti-ADAR antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ADAR on HEK293 (1) and ADAR-hIgGFc transfected HEK293 (2) cell lysate using anti-ADAR antibody at 1/1,000 dilution.
Application
Fig3: ICC staining ADAR (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of Hela cells with ADAR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Application
Fig5: Flow cytometric analysis of Jurkat cells with ADAR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ADAR recombinant protein, ADAR-hIgGFc transfected HEK293 cell lysate, Hela, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
