Application
Fig1: Western blot analysis of RUNX3 on human RUNX3 recombinant protein using anti-RUNX3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RUNX3 on HEK293 (1) and RUNX3-hIgGFc transfected HEK293 (2) cell lysate using anti-RUNX3 antibody at 1/1,000 dilution.Application
Fig3: ICC staining RUNX3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-RUNX3 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-RUNX3 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with RUNX3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RUNX3 [B8-E2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acute myeloid leukemia 2 protein antibody Acute myeloid leukemia gene 2 antibody AML 2 antibody AML2 antibody CBF alpha 3 antibody CBF-alpha-3 antibody CBFA 3 antibody CBFA3 antibody Core binding factor alpha 3 subunit antibody core binding factor antibody Core binding factor runt domain alpha subunit 3 antibody Core binding factor subunit alpha 3 antibody core-binding factor antibody Core-binding factor subunit alpha-3 antibody Oncogene AML 2 antibody Oncogene AML-2 antibody PEA2 alpha C antibody PEA2-alpha C antibody PEBP2 alpha C antibody PEBP2-alpha C antibody Pebp2a3 antibody PEBP2aC antibody Polyomavirus enhancer binding protein 2 alpha C subunit antibody Polyomavirus enhancer-binding protein 2 alpha C subunit antibody runt domain alpha subunit 3 antibody runt related transcription factor 3 antibody Runt-related transcription factor 3 antibody RUNX 3 antibody Runx3 antibody RUNX3_HUMAN antibody SL3 3 enhancer factor 1 alpha C subunit antibody SL3-3 enhancer factor 1 alpha C subunit antibody SL3/AKV core binding factor alpha C subunit antibody SL3/AKV core-binding factor alpha C subunit antibody Transcription factor AML2 antibody |
| Product description | The mammalian Runt-related transcription factor (RUNX) family comprises three members, RUNX1 (also designated AML-1, PEBP2αB, CBFA2), RUNX2 (also designated AML-3, PEBP2αA, CBFA1, Osf2) and RUNX3 (also designated AML-2, PEBPαC, CBFA3), and belongs to the acute myeloid leukemia (AML) family. RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX3 is expressed in cells of hematopoietic origin, including myeloid and B-cell lines and spleen. By playing a role in controlling the growth and differentiation of gastric epithelial cells, RUNX3 is a strong candidate as a gastric cancer tumor suppressor. Specifically, hypermethylation inactivates the gene encoding RUNX3. The detection of hypermethylation at multiple regions within the RUNX3 CpG island may aid in the diagnosis and risk assessment of gastric cancer. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Acute myeloid leukemia 2 protein antibody Acute myeloid leukemia gene 2 antibody AML 2 antibody AML2 antibody CBF alpha 3 antibody CBF-alpha-3 antibody CBFA 3 antibody CBFA3 antibody Core binding factor alpha 3 subunit antibody core binding factor antibody Core binding factor runt domain alpha subunit 3 antibody Core binding factor subunit alpha 3 antibody core-binding factor antibody Core-binding factor subunit alpha-3 antibody Oncogene AML 2 antibody Oncogene AML-2 antibody PEA2 alpha C antibody PEA2-alpha C antibody PEBP2 alpha C antibody PEBP2-alpha C antibody Pebp2a3 antibody PEBP2aC antibody Polyomavirus enhancer binding protein 2 alpha C subunit antibody Polyomavirus enhancer-binding protein 2 alpha C subunit antibody runt domain alpha subunit 3 antibody runt related transcription factor 3 antibody Runt-related transcription factor 3 antibody RUNX 3 antibody Runx3 antibody RUNX3_HUMAN antibody SL3 3 enhancer factor 1 alpha C subunit antibody SL3-3 enhancer factor 1 alpha C subunit antibody SL3/AKV core binding factor alpha C subunit antibody SL3/AKV core-binding factor alpha C subunit antibody Transcription factor AML2 antibody |
|---|---|
| Molecular Weight(MW) | 44 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
| SwissProt ID | Q13761 |
|---|
Application
Fig1: Western blot analysis of RUNX3 on human RUNX3 recombinant protein using anti-RUNX3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RUNX3 on HEK293 (1) and RUNX3-hIgGFc transfected HEK293 (2) cell lysate using anti-RUNX3 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining RUNX3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-RUNX3 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-RUNX3 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with RUNX3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RUNX3 recombinant protein, RUNX3-hIgGFc transfected HEK293 cell lysate, Hela, human esophageal cancer tissue, human stomach cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
