Application
Fig1: Western blot analysis of AXIN1 on human AXIN1 recombinant protein using anti-AXIN1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of AXIN1 on HEK293 (1) and AXIN1-hIgGFc transfected HEK293 (2) cell lysate using anti-AXIN1 antibody at 1/1,000 dilution.Application
Fig3: ICC staining AXIN1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Flow cytometric analysis of Hela cells with AXIN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | AXIN1 [F1-E9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI316800 antibody AXIN antibody Axin 1 antibody Axin-1 antibody axin1 antibody AXIN1_HUMAN antibody Axis inhibition protein 1 antibody Axis Inhibitor 1 antibody Fu antibody fused antibody Fused, mouse, homolog of antibody hAxin antibody Kb antibody Ki antibody kinky antibody knobbly antibody MGC132911 antibody MGC52315 antibody PPP1R49 antibody Protein Fused antibody Protein phosphatase 1 regulatory subunit 49 antibody |
| Product description | Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AI316800 antibody
AXIN antibody
Axin 1 antibody
Axin-1 antibody
axin1 antibody
AXIN1_HUMAN antibody
Axis inhibition protein 1 antibody
Axis Inhibitor 1 antibody
Fu antibody
fused antibody
Fused mouse homolog of antibody hAxin antibody Kb antibody Ki antibody kinky antibody knobbly antibody MGC132911 antibody MGC52315 antibody PPP1R49 antibody Protein Fused antibody Protein phosphatase 1 regulatory subunit 49 antibody |
|---|---|
| Molecular Weight(MW) | 133 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Membrane. Cell membrane.? |
| SwissProt ID | O15169 |
|---|
Application
Fig1: Western blot analysis of AXIN1 on human AXIN1 recombinant protein using anti-AXIN1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of AXIN1 on HEK293 (1) and AXIN1-hIgGFc transfected HEK293 (2) cell lysate using anti-AXIN1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining AXIN1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of Hela cells with AXIN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human AXIN1 recombinant protein, AXIN1-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
