Application
Fig1: Western blot analysis of BAFF on human BAFF recombinant protein using anti- BAFF antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of BAFF on HEK293 (1) and BAFF -hIgGFc transfected HEK293 (2) cell lysate using anti- BAFF antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of BAFF on SK-N-SH (1) and MOLT4 (2) cell lysate using anti- BAFF antibody at 1/1,000 dilution.Application
Fig4:ICC staining BAFF (green) and Actin filaments (red) in SMMC-7721 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Hela cells with BAFF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | BAFF [A2-C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B cell activating factor receptor antibody B-cell-activating factor receptor antibody BAFF R antibody BAFF receptor antibody BAFF-R antibody BAFFR antibody BLyS receptor 3 antibody BlySR3 antibody BR 3 antibody BR3 antibody BROMIX antibody CD 268 antibody CD268 antibody CD268 antigen antibody CVID4 antibody MGC138235 antibody OTTHUMP00000028746 antibody Prolixin antibody TNFRSF 13C antibody Tnfrsf13c antibody TR13C_HUMAN antibody Tumor necrosis factor receptor subunit member 13C antibody Tumor necrosis factor receptor superfamily member 13C antibody |
| Product description | TNF cytokine family member TALL-1 (also designated BAFF, TNFSF13B and BLyS), is a type II membrane protein that shares characteristics with other members of the tumor necrosis factor (TNF) cytokine family. TALL-1 is expressed abun-dantly in peripheral blood leukocytes, monocytes and macrophages. TALL-1 is critically involved in the regulation of infections, inflammation, autoimmune diseases and tissue homeostasis. TALL-1 is a potent B cell stimulatory factor which is functionally involved in B cell proliferation and may be a primary mediator in B cell-associated autoimmune diseases. The C-terminal extracellular domain has b jelly roll topography and is important in ligand trimerization. The binding of the ligand to its respective receptor induces oligomerization, initiating downstream signaling events. Intrinsic to oligomerization is the formation of the receptor binding site at the junction between neighboring subunits, creating a multivalent ligand. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | B cell activating factor receptor antibody B-cell-activating factor receptor antibody BAFF R antibody BAFF receptor antibody BAFF-R antibody BAFFR antibody BLyS receptor 3 antibody BlySR3 antibody BR 3 antibody BR3 antibody BROMIX antibody CD 268 antibody CD268 antibody CD268 antigen antibody CVID4 antibody MGC138235 antibody OTTHUMP00000028746 antibody Prolixin antibody TNFRSF 13C antibody Tnfrsf13c antibody TR13C_HUMAN antibody Tumor necrosis factor receptor subunit member 13C antibody Tumor necrosis factor receptor superfamily member 13C antibody |
|---|---|
| Molecular Weight(MW) | 31 kDa |
| Cellular Localization | Secreted and Cell membrane. |
| SwissProt ID | Q9Y275 |
|---|
Application
Fig1: Western blot analysis of BAFF on human BAFF recombinant protein using anti- BAFF antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of BAFF on HEK293 (1) and BAFF -hIgGFc transfected HEK293 (2) cell lysate using anti- BAFF antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of BAFF on SK-N-SH (1) and MOLT4 (2) cell lysate using anti- BAFF antibody at 1/1,000 dilution.
Application
Fig4:ICC staining BAFF (green) and Actin filaments (red) in SMMC-7721 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Hela cells with BAFF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human BAFF recombinant protein, BAFF -hIgGFc transfected HEK293 cell lysate, SK-N-SH, MOLT4, SMMC-7721, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
