Application
Fig1: Western blot analysis of BMP2 on human BMP2 recombinant protein using anti-BMP2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of BMP2 on HEK293 (1) and BMP2-hIgGFc transfected HEK293 (2) cell lysate using anti-BMP2 antibody at 1/1,000 dilution.Application
Fig3: ICC staining BMP2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining BMP2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Hela cells with BMP2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | BMP2 [G12-E10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BDA2 antibody BMP-2 antibody BMP-2A antibody Bmp2 antibody BMP2_HUMAN antibody BMP2A antibody Bone morphogenetic protein 2 antibody Bone morphogenetic protein 2A antibody |
| Product description | Induces cartilage and bone formation . Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | BDA2 antibody
BMP-2 antibody
BMP-2A antibody Bmp2 antibody BMP2_HUMAN antibody BMP2A antibody Bone morphogenetic protein 2 antibody Bone morphogenetic protein 2A antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P12643 |
|---|
Application
Fig1: Western blot analysis of BMP2 on human BMP2 recombinant protein using anti-BMP2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of BMP2 on HEK293 (1) and BMP2-hIgGFc transfected HEK293 (2) cell lysate using anti-BMP2 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining BMP2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining BMP2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Hela cells with BMP2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human BMP2 recombinant protein, BMP2-hIgGFc transfected HEK293 cell lysate, Hela, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
