Application
Fig1: Western blot analysis of Rab5a on human Rab5a recombinant protein using anti-Rab5a antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Rab5a on HEK293 (1) and Rab5a-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab5 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of Rab5a on Hela (1) and K562 (2) cell lysate using anti-Rab5a antibody at 1/1,000 dilution.Application
Fig4:ICC staining Rab5a (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Rab5a antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human duodenum tissue using anti-Rab5a antibody. Counter stained with hematoxylin.Application
Fig7:Flow cytometric analysis of Hela cells with Rab5a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | Rab5a [C11-H5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB 5 antibody RAB 5A antibody RAB5A antibody RAB5A member RAS oncogene family antibody RAB5A_HUMAN antibody RAS associated protein RAB5A antibody Ras related protein Rab 5A antibody Ras-related protein Rab-5A antibody |
| Product description | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, RAP, Ral/Rec and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves, at each stage, the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | RAB 5 antibody RAB 5A antibody RAB5A antibody RAB5A member RAS oncogene family antibody RAB5A_HUMAN antibody RAS associated protein RAB5A antibody Ras related protein Rab 5A antibody Ras-related protein Rab-5A antibody |
|---|---|
| Molecular Weight(MW) | 24 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P20339 |
|---|
Application
Fig1: Western blot analysis of Rab5a on human Rab5a recombinant protein using anti-Rab5a antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Rab5a on HEK293 (1) and Rab5a-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab5 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of Rab5a on Hela (1) and K562 (2) cell lysate using anti-Rab5a antibody at 1/1,000 dilution.
Application
Fig4:ICC staining Rab5a (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Rab5a antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human duodenum tissue using anti-Rab5a antibody. Counter stained with hematoxylin.
Application
Fig7:Flow cytometric analysis of Hela cells with Rab5a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human Rab5a recombinant protein, Rab5a-hIgGFc transfected HEK293 cell lysate, Hela, K562, human duodenum tissue, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
