Application
Fig1: Western blot analysis of MET on human MET recombinant protein using anti-MET antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of MET on HEK293 (1) and MET-hIgGFc transfected HEK293 (2) cell lysate using anti-MET antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of MET on different cell lysate using anti-MET antibody at 1/1,000 dilution. Positive control： Lane1: ?A549 Lane2: COS7 Lane3:Hela Lane4: HEK293 Lane5: HepG2 Lane6:A431Application
Fig4:ICC staining MET (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-MET antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of MCF-7 cells with MET antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | MET [C4-E9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AUTS9 antibody c met antibody D249 antibody Hepatocyte growth factor receptor antibody HGF antibody HGF receptor antibody HGF/SF receptor antibody HGFR antibody MET antibody Met proto oncogene tyrosine kinase antibody MET proto oncogene, receptor tyrosine kinase antibody Met proto-oncogene (hepatocyte growth factor receptor) antibody Met proto-oncogene antibody Met protooncogene antibody MET_HUMAN antibody Oncogene MET antibody Par4 antibody Proto-oncogene c-Met antibody RCCP2 antibody Scatter factor receptor antibody SF receptor antibody Tyrosine-protein kinase Met antibody |
| Product description | The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity. Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines. The c-Met gene product has been identified as the cell-surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 2mg/ml |
| Alternative Names | AUTS9 antibody
c met antibody
D249 antibody
Hepatocyte growth factor receptor antibody
HGF antibody
HGF receptor antibody
HGF/SF receptor antibody
HGFR antibody
MET antibody
Met proto oncogene tyrosine kinase antibody
MET proto oncogene receptor tyrosine kinase antibody Met proto-oncogene (hepatocyte growth factor receptor) antibody Met proto-oncogene antibody Met protooncogene antibody MET_HUMAN antibody Oncogene MET antibody Par4 antibody Proto-oncogene c-Met antibody RCCP2 antibody Scatter factor receptor antibody SF receptor antibody Tyrosine-protein kinase Met antibody |
|---|---|
| Molecular Weight(MW) | 155 kDa |
| Cellular Localization | Membrane. Single-pass type I membrane protein. |
| SwissProt ID | P08581 |
|---|
Application
Fig1: Western blot analysis of MET on human MET recombinant protein using anti-MET antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of MET on HEK293 (1) and MET-hIgGFc transfected HEK293 (2) cell lysate using anti-MET antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of MET on different cell lysate using anti-MET antibody at 1/1,000 dilution. Positive control： Lane1: ?A549 Lane2: COS7 Lane3:Hela Lane4: HEK293 Lane5: HepG2 Lane6:A431
Application
Fig4:ICC staining MET (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-MET antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of MCF-7 cells with MET antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human MET recombinant protein, MET-hIgGFc transfected HEK293 cell lysate, A549, COS7, Hela, HEK293, HepG2, A431, MCF-7, human endometrial cancer. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
