Application
Fig1: Western blot analysis of ATP2A1 on human ATP2A1 recombinant protein using anti-ATP2A1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ATP2A1 on HEK293 (1) and ATP2A1-hIgGFc transfected HEK293 (2) cell lysate using anti-ATP2A1 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of ATP2A1 on different cell lysate using anti-ATP2A1 antibody at 1/1,000 dilution.? Positive control： Lane1: C2C12 Lane2: COS7 Lane3: Hela Lane4: K562 Lane5: JurkatApplication
Fig4:Flow cytometric analysis of Hela cells with ATP2A1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ATP2A1 [H6-D1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle. The SERCAs mediate Ca2+ uptake into intracellular stores. SERCA-mediated Ca2+ uptake induces and maintains muscular relaxation. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue-dependent processing which is responsible for the generation of the SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle, and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells. SERCA2 plays an important part in regulating cardiac contractile function. SERCA3 is an isoform expressed in several cell types including platelets, lymphoid cells and mast cells. SERCA1, SERCA2 and SERCA3 all undergo alternative splicing. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 2mg/ml |
| Alternative Names | fast twitch skeletal muscle isoform antibody
AT2A1_HUMAN antibody
ATP2A antibody
ATP2A1 antibody
ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
ATPase Ca++ transporting fast twitch 1 antibody
ATPase Ca(2+)-transporting fast twitch 1 antibody Calcium pump 1 antibody Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody Calcium-transporting ATPase sarcoplasmic reticulum type antibody EC 3.6.3.8 antibody Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody Fast skeletal muscle SR calcium ATPase antibody OTTHUMP00000162561 antibody OTTHUMP00000162562 antibody Sarcoendoplasmic reticulum calcium ATPase antibody Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody SERCA 1 antibody SERCA1 antibody SERCA1 truncated isoform included antibody SR Ca(2+) ATPase 1 antibody SR Ca(2+)-ATPase 1 antibody |
|---|---|
| Molecular Weight(MW) | 110 kDa |
Application
Fig1: Western blot analysis of ATP2A1 on human ATP2A1 recombinant protein using anti-ATP2A1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ATP2A1 on HEK293 (1) and ATP2A1-hIgGFc transfected HEK293 (2) cell lysate using anti-ATP2A1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of ATP2A1 on different cell lysate using anti-ATP2A1 antibody at 1/1,000 dilution.? Positive control： Lane1: C2C12 Lane2: COS7 Lane3: Hela Lane4: K562 Lane5: Jurkat
Application
Fig4:Flow cytometric analysis of Hela cells with ATP2A1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ATP2A1 recombinant protein, ATP2A1-hIgGFc transfected HEK293 cell lysate, C2C12, COS7, Hela, K562, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
