Application
Fig1: Western blot analysis of UCP3 on human UCP3 recombinant protein using anti-UCP3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of UCP3 on HEK293 (1) and UCP3-hIgGFc transfected HEK293 (2) cell lysate using anti-UCP3 antibody at 1/1,000 dilution.Application
Fig3:ICC staining UCP3 (green) and Actin filaments (red) in HL-7702 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | UCP3 [C6-B8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitochondrial uncoupling protein 3 antibody SLC25A9 antibody Solute carrier family 25 member 9 antibody UCP 3 antibody UCP3 antibody UCP3_HUMAN antibody Uncoupling protein 3 antibody Uncoupling protein 3 mitochondrial proton carrier antibody |
| Product description | UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:500-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Mitochondrial uncoupling protein 3 antibody SLC25A9 antibody Solute carrier family 25 member 9 antibody UCP 3 antibody UCP3 antibody UCP3_HUMAN antibody Uncoupling protein 3 antibody Uncoupling protein 3 mitochondrial proton carrier antibody |
|---|---|
| Molecular Weight(MW) | 34.2 kDa |
| Cellular Localization | Mitochondrion inner membrane. |
| SwissProt ID | P55916 |
|---|
Application
Fig1: Western blot analysis of UCP3 on human UCP3 recombinant protein using anti-UCP3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of UCP3 on HEK293 (1) and UCP3-hIgGFc transfected HEK293 (2) cell lysate using anti-UCP3 antibody at 1/1,000 dilution.
Application
Fig3:ICC staining UCP3 (green) and Actin filaments (red) in HL-7702 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Human UCP3 recombinant protein, UCP3-hIgGFc transfected HEK293 cell lysate, HL-7702. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
