Application
Fig1: Western blot analysis of DDX5 on human DDX5 recombinant protein using anti-DDX5 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of DDX5 on HEK293 (1) and DDX5-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX5 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution. Positive control： Lane1: HT-29 Lane2: Hela Lane3: NIH/3T3 Lane4: COS7 Lane5: SW620 Lane6: Jurkat Lane7: A431 Lane8: MCF-7Application
Fig4:Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-DDX5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-DDX5 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with DDX5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | DDX5 [C12-F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP dependent RNA helicase DDX5 antibody DDX 5 antibody Ddx5 antibody DDX5_HUMAN antibody DEAD (Asp Glu Ala Asp) box helicase 5 antibody DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody DEAD box 5 antibody DEAD box protein 5 antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody G17P1 antibody HELR antibody HLR1 antibody HUMP68 antibody P68 antibody p68 RNA helicase antibody Probable ATP dependent RNA helicase DDX5 antibody Probable ATP-dependent RNA helicase DDX5 antibody RNA helicase p68 antibody |
| Product description | p68 RNA helicase (also named DDX5) is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 2mg/ml |
| Alternative Names | ATP dependent RNA helicase DDX5 antibody
DDX 5 antibody
Ddx5 antibody
DDX5_HUMAN antibody
DEAD (Asp Glu Ala Asp) box helicase 5 antibody
DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
DEAD box 5 antibody
DEAD box protein 5 antibody
DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase 68kD) antibody G17P1 antibody HELR antibody HLR1 antibody HUMP68 antibody P68 antibody p68 RNA helicase antibody Probable ATP dependent RNA helicase DDX5 antibody Probable ATP-dependent RNA helicase DDX5 antibody RNA helicase p68 antibody |
|---|---|
| Molecular Weight(MW) | 69 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of DDX5 on human DDX5 recombinant protein using anti-DDX5 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DDX5 on HEK293 (1) and DDX5-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX5 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution. Positive control： Lane1: HT-29 Lane2: Hela Lane3: NIH/3T3 Lane4: COS7 Lane5: SW620 Lane6: Jurkat Lane7: A431 Lane8: MCF-7
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with DDX5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human DDX5 recombinant protein, DDX5-hIgGFc transfected HEK293 cell lysate, HT-29, Hela, NIH/3T3, COS7, SW620, Jurkat, A431, MCF-7, human bladder cancer, human esophageal cancer. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
