Application
Fig1: Western blot analysis of GLI1 on human GLI1 recombinant protein using anti-GLI1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of GLI1 on HEK293 (1) and GLI1-hIgGFc transfected HEK293 (2) cell lysate using anti-GLI1 antibody at 1/1,000 dilution.Application
Fig3: ICC staining GLI1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-GLI1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-GLI1 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of HepG2 cells with GLI1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | GLI1 [F8-B9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gli 1 antibody GLI antibody GLI family zinc finger 1 antibody GLI Kruppel family member 1 antibody gli1 antibody GLI1_HUMAN antibody Glioma associated oncogene 1 antibody Glioma associated oncogene homolog 1 (zinc finger protein) antibody Glioma associated oncogene homolog antibody Glioma-associated oncogene antibody Oncogene GLI antibody Zfp 5 antibody Zfp5 antibody Zinc finger protein GLI 1 antibody Zinc finger protein GLI1 antibody |
| Product description | Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Gli 1 antibody GLI antibody GLI family zinc finger 1 antibody GLI Kruppel family member 1 antibody gli1 antibody GLI1_HUMAN antibody Glioma associated oncogene 1 antibody Glioma associated oncogene homolog 1 (zinc finger protein) antibody Glioma associated oncogene homolog antibody Glioma-associated oncogene antibody Oncogene GLI antibody Zfp 5 antibody Zfp5 antibody Zinc finger protein GLI 1 antibody Zinc finger protein GLI1 antibody |
|---|---|
| Molecular Weight(MW) | 118 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | P08151 |
|---|
Application
Fig1: Western blot analysis of GLI1 on human GLI1 recombinant protein using anti-GLI1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of GLI1 on HEK293 (1) and GLI1-hIgGFc transfected HEK293 (2) cell lysate using anti-GLI1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining GLI1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-GLI1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-GLI1 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HepG2 cells with GLI1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human GLI1 recombinant protein, GLI1-hIgGFc transfected HEK293 cell lysate, Hela, HepG2, human ovarian cancer tissue, human esophageal cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
