Application
Fig1: Western blot analysis of RIPK4 on?human RIPK4 recombinant protein using anti-RIPK4 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RIPK4 on HEK293 (1) and RIPK4-hIgGFc transfected HEK293 (2) cell lysate using anti-RIPK4 antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of SK-N-SH cells with RIPK4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RIPK4 [D9-F7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANKK 2 antibody ANKK2 antibody ANKRD 3 antibody ANKRD3 antibody Ankyrin repeat domain 3 antibody ankyrin repeat domain containing protein 3 antibody Ankyrin repeat domain protein 3 antibody DIK antibody MGC129992 antibody MGC129993 antibody PKC delta interacting protein kinase antibody PKK antibody protein kinase C-associated kinase antibody Receptor interacting serine threonine kinase 4 antibody Receptor interacting serine/threonine protein kinase 4 antibody RIP 4 antibody RIP4 antibody RIPK 4 antibody Serine/threonine protein kinase ANKRD3 antibody |
| Product description | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. RIP4, also known as RIPK4 (receptor-interacting serine/threonine-protein kinase 4), PKK (PKC δ-interacting protein kinase), DIK, ANKK2 or ANKRD3 (ankyrin repeat domain-containing protein 3), is a peripheral membrane protein that belongs to the TKL Ser/Thr protein kinase family and functions as a receptor-interacting protein (RIP) that modulates epidermal growth and differentiation. RIPs interact with the intracellular domain of tumor necrosis factor receptors (TNFRs) and facilitate downstream signaling and apoptosis induction. RIP4 contains ten ankyrin-repeats and one protein kinase domain, and participates in the activation of NFκB. Two isoforms exist due to alternative splicing events. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ANKK 2 antibody ANKK2 antibody ANKRD 3 antibody ANKRD3 antibody Ankyrin repeat domain 3 antibody ankyrin repeat domain containing protein 3 antibody Ankyrin repeat domain protein 3 antibody DIK antibody MGC129992 antibody MGC129993 antibody PKC delta interacting protein kinase antibody PKK antibody protein kinase C-associated kinase antibody Receptor interacting serine threonine kinase 4 antibody Receptor interacting serine/threonine protein kinase 4 antibody RIP 4 antibody RIP4 antibody RIPK 4 antibody Serine/threonine protein kinase ANKRD3 antibody |
|---|---|
| Molecular Weight(MW) | 92 kDa |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. |
| SwissProt ID | P57078 |
|---|
Application
Fig1: Western blot analysis of RIPK4 on?human RIPK4 recombinant protein using anti-RIPK4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RIPK4 on HEK293 (1) and RIPK4-hIgGFc transfected HEK293 (2) cell lysate using anti-RIPK4 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of SK-N-SH cells with RIPK4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RIPK4 recombinant protein, RIPK4-hIgGFc transfected HEK293 cell lysate, SK-N-SH. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
