Application
Fig1: Western blot analysis of Rab1b on ?human Rab1b recombinant protein using anti-Rab1b antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Rab1b on HEK293 (1) and Rab1b-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab1b antibody at 1/1,000 dilution.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-Rab1b antibody. Counter stained with hematoxylin.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-Rab1b antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with Rab1b antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | Rab1b [G2-A12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB1B antibody RAB1B member RAS oncogene family antibody RAB1B_HUMAN antibody Ras related protein Rab1B antibody Ras-related protein Rab-1B antibody Small GTP binding protein antibody |
| Product description | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, exhibits 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | RAB1B antibody RAB1B member RAS oncogene family antibody RAB1B_HUMAN antibody Ras related protein Rab1B antibody Ras-related protein Rab-1B antibody Small GTP binding protein antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Membrane. Cytoplasm. |
| SwissProt ID | Q9H0U4 |
|---|
Application
Fig1: Western blot analysis of Rab1b on ?human Rab1b recombinant protein using anti-Rab1b antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Rab1b on HEK293 (1) and Rab1b-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab1b antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-Rab1b antibody. Counter stained with hematoxylin.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-Rab1b antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with Rab1b antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human Rab1b recombinant protein, Rab1b-hIgGFc transfected HEK293 cell lysate, human bladder cancer tissue, human esophageal cancer tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
