Application
Fig1: Western blot analysis of Tlr2 on mouse Tlr2 recombinant protein using anti-Tlr2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Tlr2 on HEK293 (1) and Tlr2-hIgGFc transfected HEK293 (2) cell lysate using anti-Tlr2 antibody at 1/1,000 dilution.Application
Fig3: ICC staining Tlr2 (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | Tlr2 [F4-G5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD282 antibody CD282 antigen antibody TIL 4 antibody TIL4 antibody TLR 2 antibody TLR2 antibody TLR2_HUMAN antibody Toll like receptor 2 antibody Toll like receptor 2 precursor antibody Toll-like receptor 2 antibody Toll/interleukin 1 receptor like 4 antibody Toll/interleukin 1 receptor like protein 4 antibody Toll/interleukin receptor like protein 4 antibody Toll/interleukin-1 receptor-like protein 4 antibody |
| Product description | Six human homologs of the Drosophila Toll receptor were initially identified based on their sequence similarities and designated Toll-like receptors (TLR). Toll receptors are involved in mediating dorsoventral polarization in the developing Drosophila embryo and also participate in the host immunity. The TLR family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses. Expression of TLR receptors is highest in peripheral blood leukocytes, macrophages, and monocytes (2,7). TLR6 is highly homologous to TLR1, sharing greater than 65% sequence identity, and, like other members of TLR family, it induces NFkB signaling upon activation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:500-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CD282 antibody CD282 antigen antibody TIL 4 antibody TIL4 antibody TLR 2 antibody TLR2 antibody TLR2_HUMAN antibody Toll like receptor 2 antibody Toll like receptor 2 precursor antibody Toll-like receptor 2 antibody Toll/interleukin 1 receptor like 4 antibody Toll/interleukin 1 receptor like protein 4 antibody Toll/interleukin receptor like protein 4 antibody Toll/interleukin-1 receptor-like protein 4 antibody |
|---|---|
| Molecular Weight(MW) | 90 kDa |
| Cellular Localization | Membrane. Cytoplasmic vesicle. Membrane raft. |
| SwissProt ID | O60603 |
|---|
Application
Fig1: Western blot analysis of Tlr2 on mouse Tlr2 recombinant protein using anti-Tlr2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Tlr2 on HEK293 (1) and Tlr2-hIgGFc transfected HEK293 (2) cell lysate using anti-Tlr2 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining Tlr2 (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Mouse Tlr2 recombinant protein, Tlr2-hIgGFc transfected HEK293 cell lysate, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
