Application
Fig1: Western blot analysis of GAD2 on human GAD2 recombinant protein using anti-GAD2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of GAD2 on HEK293 (1) and GAD2-hIgGFc transfected HEK293 (2) cell lysate using anti-GAD2 antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of Hela cells with GAD2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | GAD2 [G9-D12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 65 kDa glutamic acid decarboxylase antibody DCE 2 antibody DCE2 antibody DCE2_HUMAN antibody GAD 2 antibody GAD 65 antibody GAD-2 antibody GAD-65 antibody Gad2 antibody Glutamate decarboxylase 2 (pancreas) antibody Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody Glutamate decarboxylase 2 antibody Glutamate Decarboxylase 65 antibody Glutamate decarboxylase 65 kDa isoform antibody Glutamic Acid Decarboxylase 2 antibody Glutamic Acid Decarboxylase 65 antibody MGC161605 antibody MGC161607 antibody |
| Product description | There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD-65 (also known as GAD2) and GAD-67 (also known as GAD1, GAD or SCP). GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67, the predominant GAD-67 form and the minor GAD-25 form. GAD-25 is not expressed in brain but can be found in a variety of endocrine tissues. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 65 kDa glutamic acid decarboxylase antibody DCE 2 antibody DCE2 antibody DCE2_HUMAN antibody GAD 2 antibody GAD 65 antibody GAD-2 antibody GAD-65 antibody Gad2 antibody Glutamate decarboxylase 2 (pancreas) antibody Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody Glutamate decarboxylase 2 antibody Glutamate Decarboxylase 65 antibody Glutamate decarboxylase 65 kDa isoform antibody Glutamic Acid Decarboxylase 2 antibody Glutamic Acid Decarboxylase 65 antibody MGC161605 antibody MGC161607 antibody |
|---|---|
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Cytoplasm. Cytoplasmic vesicle. |
| SwissProt ID | Q05329 |
|---|
Application
Fig1: Western blot analysis of GAD2 on human GAD2 recombinant protein using anti-GAD2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of GAD2 on HEK293 (1) and GAD2-hIgGFc transfected HEK293 (2) cell lysate using anti-GAD2 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with GAD2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human GAD2 recombinant protein, GAD2-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
