Application
Fig1: Western blot analysis of ZFP91 on ?human ZFP91 recombinant protein using anti-ZFP91 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ZFP91 on HEK293 (1) and ZFP91-hIgGFc transfected HEK293 (2) cell lysate using anti-ZFP91 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of ZFP91 on different cell lysate using anti-ZFP91 antibody at 1/1,000 dilution. Positive control： Lane1: Jurkat Lane2: A431 Lane3: HepG2 Lane4: HEK293 Lane5: A549 Lane6:PC-3Application
Fig4:ICC staining ZFP91 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-ZFP91 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human ?rectum cancer tissue using anti-ZFP91 antibody. Counter stained with hematoxylin.Application
Fig7:Flow cytometric analysis of Hela cells with ZFP91 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ZFP91 [D5-C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FKSG11 antibody HGNC:14983 antibody PZF antibody zinc finger protein 91 antibody zinc finger protein 91 homolog (mouse) antibody |
| Product description | The protein encoded by ZFP91 is a member of the zinc finger family of proteins. This protein contains C2H2 type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:100-1:200 IHC:1:100-1:500 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | FKSG11 antibody HGNC:14983 antibody PZF antibody zinc finger protein 91 antibody zinc finger protein 91 homolog (mouse) antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q96JP5 |
|---|
Application
Fig1: Western blot analysis of ZFP91 on ?human ZFP91 recombinant protein using anti-ZFP91 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ZFP91 on HEK293 (1) and ZFP91-hIgGFc transfected HEK293 (2) cell lysate using anti-ZFP91 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of ZFP91 on different cell lysate using anti-ZFP91 antibody at 1/1,000 dilution. Positive control： Lane1: Jurkat Lane2: A431 Lane3: HepG2 Lane4: HEK293 Lane5: A549 Lane6:PC-3
Application
Fig4:ICC staining ZFP91 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-ZFP91 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human ?rectum cancer tissue using anti-ZFP91 antibody. Counter stained with hematoxylin.
Application
Fig7:Flow cytometric analysis of Hela cells with ZFP91 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ZFP91 recombinant protein, ZFP91-hIgGFc transfected HEK293 cell lysate, Jurkat, A431, HepG2, HEK293, A549, PC-3,Hela, human ovarian cancer, human ?rectum cancer. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:200 IHC:1:100-1:500 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
