Application
Fig1: Western blot analysis of EIF2A on human EIF2A recombinant protein using anti-EIF2A antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of EIF2A on HEK293 (1) and EIF2A-hIgGFc transfected HEK293 (2) cell lysate using anti-EIF2A antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of EIF2A on different cell lysate using anti-EIF2A antibody at 1/1,000 dilution. Positive control： Line1: MCF-7 Line2: PC-12 Line3: HepG2 Line4: Hela Line5: Cos7 Line6: K562 Line7: Jurkat Line8: A431 Line9:Application
Fig4: ICC staining EIF2A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-EIF2A antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of HepG2 cells with EIF2A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | EIF2A [A8-A7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 65 kDa eukaryotic translation initiation factor 2A antibody CDA 02 antibody CDA02 antibody EIF 2 antibody EIF 2A antibody eIF-2A antibody EIF2 antibody eif2a antibody EIF2A_HUMAN antibody Eukaryotic translation initiation factor 2A 65kDa antibody Eukaryotic translation initiation factor 2A antibody MST089 antibody MSTP004 antibody MSTP089 antibody |
| Product description | eIF2A (eukaryotic translation initiation factor 2A), also known as CDA02, MSTP004 or MSTP089, is a 585 amino acid protein that contains three WD repeats. Expressed ubiquitously with highest expression in heart, brain, pancreas and placenta, eIF2A functions as a translation initiation factor that binds Met-tRNA and directs it to 40S ribosomal subunits. Present in the early steps of protein synthesis, eIF2A controls the binding of Met-tRNA to 40S ribosomal subunits in a codon-dependent manner, in contrast to the eIF2 complex which accomplishes the same task in a GTP-dependent manner. In addition to its role in transcription initiation, eIF2A may also act to negatively regulate the expression of specific proteins, suggesting a possible role as a transcriptional repressor. eIF2A exists as two isoforms due to alternative splicing events. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 65 kDa eukaryotic translation initiation factor 2A antibody CDA 02 antibody CDA02 antibody EIF 2 antibody EIF 2A antibody eIF-2A antibody EIF2 antibody eif2a antibody EIF2A_HUMAN antibody Eukaryotic translation initiation factor 2A 65kDa antibody Eukaryotic translation initiation factor 2A antibody MST089 antibody MSTP004 antibody MSTP089 antibody |
|---|---|
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q9BY44 |
|---|
Application
Fig1: Western blot analysis of EIF2A on human EIF2A recombinant protein using anti-EIF2A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of EIF2A on HEK293 (1) and EIF2A-hIgGFc transfected HEK293 (2) cell lysate using anti-EIF2A antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of EIF2A on different cell lysate using anti-EIF2A antibody at 1/1,000 dilution. Positive control： Line1: MCF-7 Line2: PC-12 Line3: HepG2 Line4: Hela Line5: Cos7 Line6: K562 Line7: Jurkat Line8: A431 Line9:
Application
Fig4: ICC staining EIF2A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-EIF2A antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HepG2 cells with EIF2A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human EIF2A recombinant protein, EIF2A-hIgGFc transfected HEK293 cell lysate, MCF-7, PC-12, HepG2, Hela, Cos7, K562, Jurkat, A431, NIH/3T3 cell lysate, human cervical cancer tissue, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
