Application
Fig1: Western blot analysis of DNM1L on human DNM1L recombinant protein using anti-DNM1L antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of DNM1L on HEK293 (1) and DNM1L-hIgGFc transfected HEK293 (2) cell lysate using anti-DNM1L antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of DNM1L on different cell lysate using anti-DNM1L antibody at 1/1,000 dilution. Positive control： Line1: PC-12 Line2: LNCAP Line3: NIH/3T3Application
Fig4: ICC staining DNM1L (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of HEK293 cells with DNM1L antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | DNM1L [C7-A4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DLP1 antibody dnm1l antibody DNM1L_HUMAN antibody Dnm1p/Vps1p-like protein antibody dnml1 antibody DRP1 antibody DVLP antibody Dymple antibody Dynamin 1 like antibody Dynamin family member proline-rich carboxyl-terminal domain less antibody Dynamin like protein antibody Dynamin related protein 1 antibody Dynamin-1-like protein antibody Dynamin-like protein 4 antibody Dynamin-like protein antibody Dynamin-like protein IV antibody Dynamin-related protein 1 antibody DYNIV 11 antibody EMPF antibody EMPF1 antibody FLJ41912 antibody HdynIV antibody VPS1 antibody |
| Product description | Dynamin-related protein 1 (DRP1) mediates outer mitochondrial membrane fission in mammalian cells. DRP1 is also known as Dynamin-like protein 1, (Dlp1), DVLP or Dymple. DRP1 contains the N-terminal tripartite GTP-binding domain characteristic of the Dynamin superfamily of GTPases. DRP1 exists as a T-shaped dimer which contains a head, leg and stalk. The addition of GTP induces a rearrangement of the head and stalk that generates a force that ultimately results in membrane constriction. DRP1 is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain. In the cell, DRP1 localized to the perinuclear region. In mouse brain, DRP1 is highly expressed in the cerebellum with particularly high levels in cerebellar Purkinje cells. During apoptosis, DRP1 translocates from the cytosol to mitochondria and localizes to potential sites of organelle division. Cell death is averted upon DRP inhibition, suggesting a critical role for mitochondrial fission in apoptosis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:100-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | DLP1 antibody dnm1l antibody DNM1L_HUMAN antibody Dnm1p/Vps1p-like protein antibody dnml1 antibody DRP1 antibody DVLP antibody Dymple antibody Dynamin 1 like antibody Dynamin family member proline-rich carboxyl-terminal domain less antibody Dynamin like protein antibody Dynamin related protein 1 antibody Dynamin-1-like protein antibody Dynamin-like protein 4 antibody Dynamin-like protein antibody Dynamin-like protein IV antibody Dynamin-related protein 1 antibody DYNIV 11 antibody EMPF antibody EMPF1 antibody FLJ41912 antibody HdynIV antibody VPS1 antibody |
|---|---|
| Molecular Weight(MW) | 82 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of DNM1L on human DNM1L recombinant protein using anti-DNM1L antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DNM1L on HEK293 (1) and DNM1L-hIgGFc transfected HEK293 (2) cell lysate using anti-DNM1L antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of DNM1L on different cell lysate using anti-DNM1L antibody at 1/1,000 dilution. Positive control： Line1: PC-12 Line2: LNCAP Line3: NIH/3T3
Application
Fig4: ICC staining DNM1L (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of HEK293 cells with DNM1L antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human DNM1L recombinant protein, DNM1L-hIgGFc transfected HEK293 cell lysate, PC-12, LNCAP, NIH/3T3 cell lysate, Hela, HEK293. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
