Application
Fig1: Western blot analysis of PAK3 on human PAK3 recombinant protein using anti-PAK3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PAK3 on HEK293 (1) and PAK3-hIgGFc transfected HEK293 (2) cell lysate using anti-PAK3 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of PAK3 on different cell lysate using anti-PAK3 antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: SK-N-SH Line3: T47D Line4: COS7 Line5: HepG2| Product Name | PAK3 [F11-G8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Beta PAK antibody bPAK antibody CDKN1A antibody hPAK3 antibody Mental retardation X linked 30 antibody MRX30 antibody MRX47 antibody Oligophrenin 3 antibody OPHN3 antibody p21 (CDKN1A) activated kinase 3 antibody p21 activated kinase 3 antibody p21 CDKN1A activated kinase 3 antibody P21 protein (Cdc42/Rac) activated kinase 3 antibody PAK-3 antibody PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 antibody PAK3beta antibody Pak65alpha antibody Pak65beta antibody Serine threonine protein kinase PAK 3 antibody Stk4 antibody |
| Product description | Three isoforms of serine/threonine kinases, designated aPAK p68, bPAK p65 and gPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The a, b and gPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is Mek kinase, an upstream effector of Mek4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 2mg/ml |
| Alternative Names | Beta PAK antibody bPAK antibody CDKN1A antibody hPAK3 antibody Mental retardation X linked 30 antibody MRX30 antibody MRX47 antibody Oligophrenin 3 antibody OPHN3 antibody p21 (CDKN1A) activated kinase 3 antibody p21 activated kinase 3 antibody p21 CDKN1A activated kinase 3 antibody P21 protein (Cdc42/Rac) activated kinase 3 antibody PAK-3 antibody PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 antibody PAK3beta antibody Pak65alpha antibody Pak65beta antibody Serine threonine protein kinase PAK 3 antibody Stk4 antibody |
|---|---|
| Molecular Weight(MW) | 62 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | O75914 |
|---|
Application
Fig1: Western blot analysis of PAK3 on human PAK3 recombinant protein using anti-PAK3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PAK3 on HEK293 (1) and PAK3-hIgGFc transfected HEK293 (2) cell lysate using anti-PAK3 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PAK3 on different cell lysate using anti-PAK3 antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: SK-N-SH Line3: T47D Line4: COS7 Line5: HepG2| Positive Control | Human PAK3 recombinant protein, PAK3-hIgGFc transfected HEK293 cell lysate, Hela, SK-N-SH, T47D, COS7, HepG2 cell lysate. |
|---|---|
| Application Notes | WB:1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
