Application
Fig1: Western blot analysis of ARFGAP1 on human ARFGAP1 recombinant protein using anti-ARFGAP1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ARFGAP1 on HEK293 (1) and ARFGAP1-hIgGFc transfected HEK293 (2) cell lysate using anti-ARFGAP1 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of ARFGAP1 on different cell lysate using anti-ARFGAP1 antibody at 1/1,000 dilution. Positive control： Line1: MOLT4 Line2: C2C12 Line3: HepG2 Line4: MCF-7 Line5: LncapApplication
Fig4: Flow cytometric analysis of HepG2 cells with ARFGAP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ARFGAP1 [E2-C4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP ribosylation factor 1 GTPase activating protein antibody ADP ribosylation factor GTPase activating protein 1 antibody ADP-ribosylation factor 1 GTPase-activating protein antibody ADP-ribosylation factor GTPase-activating protein 1 antibody ARF GAP 1 antibody ARF1 directed GTPase activating protein antibody ARF1 GAP antibody ARF1-directed GTPase-activating protein antibody ARF1GAP antibody ARFG1_HUMAN antibody Arfgap1 antibody GAP protein antibody HRIHFB2281 antibody |
| Product description | GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity). |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | ADP ribosylation factor 1 GTPase activating protein antibody ADP ribosylation factor GTPase activating protein 1 antibody ADP-ribosylation factor 1 GTPase-activating protein antibody ADP-ribosylation factor GTPase-activating protein 1 antibody ARF GAP 1 antibody ARF1 directed GTPase activating protein antibody ARF1 GAP antibody ARF1-directed GTPase-activating protein antibody ARF1GAP antibody ARFG1_HUMAN antibody Arfgap1 antibody GAP protein antibody HRIHFB2281 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm. Golgi apparatus. Associates with the Golgi complex. |
Application
Fig1: Western blot analysis of ARFGAP1 on human ARFGAP1 recombinant protein using anti-ARFGAP1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ARFGAP1 on HEK293 (1) and ARFGAP1-hIgGFc transfected HEK293 (2) cell lysate using anti-ARFGAP1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of ARFGAP1 on different cell lysate using anti-ARFGAP1 antibody at 1/1,000 dilution. Positive control： Line1: MOLT4 Line2: C2C12 Line3: HepG2 Line4: MCF-7 Line5: Lncap
Application
Fig4: Flow cytometric analysis of HepG2 cells with ARFGAP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ARFGAP1 recombinant protein, ARFGAP1-hIgGFc transfected HEK293 cell lysate, MOLT4, C2C12, HepG2, MCF-7, Lncap cell lysate, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
