Application
Fig1: Western blot analysis of ESR1 on human ESR1 recombinant protein using anti-ESR1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ESR1 on HEK293 (1) and ESR1-hIgGFc transfected HEK293 (2) cell lysate using anti-ESR1 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of ESR1 on different cell lysate using anti-ESR1 antibody at 1/1,000 dilution. Positive control： Line1: MOLT4 Line2: Raji Line3: MCF-7 Line4: T47D Line5: SK-Br-3 Line6: Hela Line7: C6Application
Fig4: ICC staining ESR1 (green) and Actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-ESR1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-ESR1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with ESR1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ESR1 [E8-A6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686N23123 antibody ER alpha antibody ER antibody ER-alpha antibody Era antibody ESR antibody ESR1 antibody ESR1_HUMAN antibody ESRA antibody Estradiol receptor antibody Estrogen nuclear receptor alpha antibody Estrogen receptor 1 antibody Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody Estrogen receptor alpha antibody Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody Estrogen receptor alpha delta 4 +49 isoform antibody Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody Estrogen receptor alpha E1 E2 1 2 antibody Estrogen receptor alpha E1 N2 E2 1 2 antibody Estrogen receptor antibody ESTRR antibody NR3A1 antibody Nuclear receptor subfamily 3 group A member 1 antibody |
| Product description | Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | DKFZp686N23123 antibody
ER alpha antibody
ER antibody
ER-alpha antibody
Era antibody
ESR antibody
ESR1 antibody
ESR1_HUMAN antibody
ESRA antibody
Estradiol receptor antibody
Estrogen nuclear receptor alpha antibody
Estrogen receptor 1 antibody
Estrogen receptor alpha 3* 4 5 6 7*/822 isoform antibody Estrogen receptor alpha antibody Estrogen receptor alpha delta 3* 4 5 6 7* 8*/941 isoform antibody Estrogen receptor alpha delta 3* 4 5 6 7*/819 2 isoform antibody Estrogen receptor alpha delta 4 +49 isoform antibody Estrogen receptor alpha delta 4* 5 6 7*/654 isoform antibody Estrogen receptor alpha delta 4* 5 6 7 8*/901 isoform antibody Estrogen receptor alpha E1 E2 1 2 antibody Estrogen receptor alpha E1 N2 E2 1 2 antibody Estrogen receptor antibody ESTRR antibody NR3A1 antibody Nuclear receptor subfamily 3 group A member 1 antibody |
|---|---|
| Molecular Weight(MW) | 66 kDa |
| Cellular Localization | Nucleus, cytoplasm. |
Application
Fig1: Western blot analysis of ESR1 on human ESR1 recombinant protein using anti-ESR1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ESR1 on HEK293 (1) and ESR1-hIgGFc transfected HEK293 (2) cell lysate using anti-ESR1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of ESR1 on different cell lysate using anti-ESR1 antibody at 1/1,000 dilution. Positive control： Line1: MOLT4 Line2: Raji Line3: MCF-7 Line4: T47D Line5: SK-Br-3 Line6: Hela Line7: C6
Application
Fig4: ICC staining ESR1 (green) and Actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-ESR1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-ESR1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with ESR1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ESR1 recombinant protein, ESR1-hIgGFc transfected HEK293 cell lysate, MOLT4, Raji, MCF-7, T47D, SK-Br-3, Hela, C6 cell lysate, Hela, human breast cancer tissue, human esophageal cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
