Application
Fig1: Western blot analysis of PRKAG3 on human PRKAG3 recombinant protein using anti-PRKAG3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PRKAG3 on HEK293 (1) and PRKAG3-hIgGFc transfected HEK293 (2) cell lysate using anti-PRKAG3 antibody at 1/1,000 dilution.Application
Fig3: ICC staining PRKAG3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum tissue using anti-PRKAG3 antibody. Counter stained with hematoxylin.| Product Name | PRKAG3 [A7-C7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5 AMP activated protein kinase subunit gamma 3 antibody 5''-AMP-activated protein kinase subunit gamma-3 antibody AAKG3_HUMAN antibody AMPK gamma 3 chain antibody AMPK gamma3 antibody AMPK subunit gamma-3 antibody AMPKG3 antibody PRKAG3 antibody Protein kinase AMP activated gamma 3 non catalytic subunit antibody |
| Product description | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enz |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 5 AMP activated protein kinase subunit gamma 3 antibody 5''-AMP-activated protein kinase subunit gamma-3 antibody AAKG3_HUMAN antibody AMPK gamma 3 chain antibody AMPK gamma3 antibody AMPK subunit gamma-3 antibody AMPKG3 antibody PRKAG3 antibody Protein kinase AMP activated gamma 3 non catalytic subunit antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cytosol, extracellular space, nucleoplasm, nucleotide-activated protein kinase complex. |
| SwissProt ID | Q9UGI9 |
|---|
Application
Fig1: Western blot analysis of PRKAG3 on human PRKAG3 recombinant protein using anti-PRKAG3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PRKAG3 on HEK293 (1) and PRKAG3-hIgGFc transfected HEK293 (2) cell lysate using anti-PRKAG3 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining PRKAG3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum tissue using anti-PRKAG3 antibody. Counter stained with hematoxylin.| Positive Control | Human PRKAG3 recombinant protein, PRKAG3-hIgGFc transfected HEK293 cell lysate, ?Hela, human rectum tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
