Application
Fig1: Western blot analysis of ATG2A on human ATG2A recombinant protein using anti-ATG2A antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ATG2A on HEK293 (1) and ATG2A-hIgGFc transfected HEK293 (2) cell lysate using anti-ATG2A antibody at 1/1,000 dilution.Application
Fig3: ICC staining ATG2A (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of K562 cells with ATG2A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ATG2A [D4-E6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1810013C15Rik antibody A830054M12 antibody ATG2 autophagy related 2 homolog A (S. cerevisiae) antibody ATG2A antibody ATG2A_HUMAN antibody Autophagy-related protein 2 homolog A antibody BC023754 antibody KIAA0404 antibody MGC117153 antibody MGC67447 antibody mKIAA0404 antibody |
| Product description | Atg2A (TG2 autophagy related 2 homolog A) is a 1,938 amino acid protein that belongs to the ATG2 family and may play a role in vesicle assembly. Encoded by a gene that maps to human chromosome 11q13.1, Atg2A is conserved in chimpanzee, dog, cow, mouse and rat, and exists as four alternatively spliced isoforms. Undetected in adult tissues, including heart, brain, placenta, lung, liver and skeletal muscle, Atg2A regulation may act as a distinct indicator of autophagic programmed cell death. Atg2A is upregulated in both etoposide- and doxorubicin-induced apoptosis of HeLa cells, suggesting that Atg2A functions as a novel biomarker of topoisomerase II inhibitor-mediated apoptosis. Atg2A associates with Atg2B, indicating that these two related proteins also functionally interact. Atg2A frameshift mutations are linked to gastric and colorectal carcinomas with high microsatellite instability and may contribute to cancer development by deregulating the autophagy process. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 1810013C15Rik antibody A830054M12 antibody ATG2 autophagy related 2 homolog A (S. cerevisiae) antibody ATG2A antibody ATG2A_HUMAN antibody Autophagy-related protein 2 homolog A antibody BC023754 antibody KIAA0404 antibody MGC117153 antibody MGC67447 antibody mKIAA0404 antibody |
|---|---|
| Molecular Weight(MW) | 213 kDa |
| Cellular Localization | Preautophagosomal structure membrane, lipid droplet. |
| SwissProt ID | Q2TAZ0 |
|---|
Application
Fig1: Western blot analysis of ATG2A on human ATG2A recombinant protein using anti-ATG2A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ATG2A on HEK293 (1) and ATG2A-hIgGFc transfected HEK293 (2) cell lysate using anti-ATG2A antibody at 1/1,000 dilution.
Application
Fig3: ICC staining ATG2A (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of K562 cells with ATG2A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ATG2A recombinant protein, ATG2A-hIgGFc transfected HEK293 cell lysate, Hela, K562. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
