Application
Fig1: Western blot analysis of ABCG5 on human ABCG5 recombinant protein using anti-ABCG5 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ABCG5 on HEK293 (1) and ABCG5-hIgGFc transfected HEK293 (2) cell lysate using anti-ABCG5 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of ABCG5 on different cell lysate using anti-ABCG5 antibody at 1/1,000 dilution. Positive control： Line1: HL7702 Line2: Raji Line3: JurkatApplication
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-ABCG5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-ABCG5 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of A549 cells with ABCG5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ABCG5 [E10-B5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Abcg5 antibody ABCG5_HUMAN antibody ATP binding cassette sub family G (WHITE) member 5 (sterolin 1) antibody ATP binding cassette sub family G member 5 antibody ATP-binding cassette sub-family G member 5 antibody ATP-binding cassette, sub-family G (WHITE), member 5 antibody Sterolin 1 antibody Sterolin-1 antibody STSL antibody |
| Product description | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:200-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Abcg5 antibody
ABCG5_HUMAN antibody
ATP binding cassette sub family G (WHITE) member 5 (sterolin 1) antibody
ATP binding cassette sub family G member 5 antibody
ATP-binding cassette sub-family G member 5 antibody
ATP-binding cassette sub-family G (WHITE) member 5 antibody Sterolin 1 antibody Sterolin-1 antibody STSL antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q9H222 |
|---|
Application
Fig1: Western blot analysis of ABCG5 on human ABCG5 recombinant protein using anti-ABCG5 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ABCG5 on HEK293 (1) and ABCG5-hIgGFc transfected HEK293 (2) cell lysate using anti-ABCG5 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of ABCG5 on different cell lysate using anti-ABCG5 antibody at 1/1,000 dilution. Positive control： Line1: HL7702 Line2: Raji Line3: Jurkat
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-ABCG5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-ABCG5 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A549 cells with ABCG5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ABCG5 recombinant protein, ABCG5-hIgGFc transfected HEK293 cell lysate, HL7702, Raji, Jurkat cell lysate, human cervical cancer tissue, human rectum cancer tissue, A549. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:200-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
