Application
Fig1: Western blot analysis of GPNMB on human GPNMB recombinant protein using anti-GPNMB antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of GPNMB on HEK293 (1) and GPNMB-hIgGFc transfected HEK293 (2) cell lysate using anti-GPNMB antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of GPNMB on PANC-1 and PC-3 cell lysate using anti-GPNMB antibody at 1/1,000 dilution.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-GPNMB antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-GPNMB antibody. Counter stained with hematoxylin.| Product Name | GPNMB [E5-C10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glycoprotein (transmembrane) nmb antibody Glycoprotein nmb antibody Glycoprotein nmb like protein antibody Gpnmb antibody GPNMB_HUMAN antibody HGFIN antibody NMB antibody Osteoactivin antibody Transmembrane glycoprotein antibody Transmembrane glycoprotein HGFIN antibody Transmembrane glycoprotein NMB antibody |
| Product description | Transmembrane glycoprotein NMB (GPNMB), also designated hematopoietic growth factor inducible neurokinin-1 (HGFIN), is a single-pass type I membrane protein. Belonging to the Pmel-17/NMB family of proteins, GPNMB acts as a melanogenic enzyme. GPNMB expression is not restricted to cells of melanocytic lineage and is highest in poorly metastatic melanoma cell lines. There is no expression of GPNMB in highly metastatic melanoma cell lines. GPNMB may play a important role in lymphohematopoietic stem cell maturation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 IHC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Glycoprotein (transmembrane) nmb antibody Glycoprotein nmb antibody Glycoprotein nmb like protein antibody Gpnmb antibody GPNMB_HUMAN antibody HGFIN antibody NMB antibody Osteoactivin antibody Transmembrane glycoprotein antibody Transmembrane glycoprotein HGFIN antibody Transmembrane glycoprotein NMB antibody |
|---|---|
| Molecular Weight(MW) | 64 kDa |
| Cellular Localization | Membrane. Melanosome. |
| SwissProt ID | Q14956 |
|---|
Application
Fig1: Western blot analysis of GPNMB on human GPNMB recombinant protein using anti-GPNMB antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of GPNMB on HEK293 (1) and GPNMB-hIgGFc transfected HEK293 (2) cell lysate using anti-GPNMB antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of GPNMB on PANC-1 and PC-3 cell lysate using anti-GPNMB antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-GPNMB antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-GPNMB antibody. Counter stained with hematoxylin.| Positive Control | Human GPNMB recombinant protein, GPNMB-hIgGFc transfected HEK293 cell lysate, PANC-1, PC-3 cell lysate, human breast cancer tissue, human esophagus cancer tissue. |
|---|---|
| Application Notes | WB:1:500 IHC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
