Application
Fig1: Western blot analysis of AEBP2 on human AEBP2 recombinant protein using anti-AEBP2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of AEBP2 on HEK293 (1) and AEBP2-hIgGFc transfected HEK293 (2) cell lysate using anti-AEBP2 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of AEBP2 on different cell lysate using anti-AEBP2 antibody at 1/1,000 dilution. Positive control： Line1: COS7 Line2: HepG2 Line3: SK-MES-1Application
Fig4: ICC staining AEBP2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of MCF-7 cells with AEBP2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | AEBP2 [B6-D7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adipocyte enhancer binding protein 2 antibody Adipocyte enhancer-binding protein 2 antibody AE (adipocyte enhancer) binding protein 2 antibody AE binding protein 2 antibody AE-binding protein 2 antibody AEBP 2 antibody AEBP2 antibody AEBP2_HUMAN antibody MGC17922 antibody Zinc finger protein AEBP2 antibody |
| Product description | AEBP2 (AE Binding Protein 2) is a Protein Coding gene. Among its related pathways are Chromatin organization and Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3. GO annotations related to this gene include RNA polymerase II core promoter proximal region sequence-specific DNA binding and transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding. DNA-binding transcriptional repressor. May interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Adipocyte enhancer binding protein 2 antibody Adipocyte enhancer-binding protein 2 antibody AE (adipocyte enhancer) binding protein 2 antibody AE binding protein 2 antibody AE-binding protein 2 antibody AEBP 2 antibody AEBP2 antibody AEBP2_HUMAN antibody MGC17922 antibody Zinc finger protein AEBP2 antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q6ZN18 |
|---|
Application
Fig1: Western blot analysis of AEBP2 on human AEBP2 recombinant protein using anti-AEBP2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of AEBP2 on HEK293 (1) and AEBP2-hIgGFc transfected HEK293 (2) cell lysate using anti-AEBP2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of AEBP2 on different cell lysate using anti-AEBP2 antibody at 1/1,000 dilution. Positive control： Line1: COS7 Line2: HepG2 Line3: SK-MES-1
Application
Fig4: ICC staining AEBP2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of MCF-7 cells with AEBP2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human AEBP2 recombinant protein, AEBP2-hIgGFc transfected HEK293 cell lysate, COS7, HepG2, SK-MES-1 cell lysate, Hela, MCF-7. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
