Application
Fig1: Western blot analysis of CD22 on human CD22 recombinant protein using anti-CD22 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CD22 on HEK293 (1) and CD22-hIgGFc transfected HEK293 (2) cell lysate using anti-CD22 antibody at 1/1,000 dilution.Application
ysis of CD22 on different cell lysate using anti-CD22 antibody at 1/1,000 dilution. Positive control： Line1: L1210 Line2: Hela Line3: HEK293 Line4: Jurkat Line5: OCM-1 Line6: A431 Line7: NIH/3T3Application
Fig4: ICC staining CD22 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CD22 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-CD22 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with CD22 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CD22 [C4-H1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B cell receptor CD22 precursor antibody B lymphocyte cell adhesion molecule antibody B-cell receptor CD22 antibody B-lymphocyte cell adhesion molecule antibody BL CAM antibody BL-CAM antibody BLCAM antibody CD 22 antibody CD22 antibody CD22 antigen antibody CD22 molecule antibody CD22 protein antibody CD22_HUMAN antibody Lectin 2 antibody Leu14 antibody Lyb8 antibody MGC130020 antibody sialic acid binding Ig like lectin 2 antibody Sialic acid binding immunoglobulin like lectin 2 antibody Sialic acid-binding Ig-like lectin 2 antibody SIGLEC 2 antibody Siglec-2 antibody SIGLEC2 antibody T cell surface antigen Leu 14 antibody T-cell surface antigen Leu-14 antibody |
| Product description | The B lymphocyte specific CD22 antigen, also designated B-lymphocyte cell adhesion molecule (BLCAM), sialic acid-binding Ig-like lectin 2 (Siglec-2) and Leu-14, is a type I integral membrane glycoprotein, structurally similar to other cell adhesion molecules (CAMs), which acts as a regulator of B cell signaling. CD22 is expressed as both a cytoplasmic and membrane protein during discrete stages of B cell lymphocyte differentiation. The cytoplasmic form of CD22, expressed early in B cell development, is a useful marker for acute lymphocytic leukemia. The membrane form of CD22 is expressed in mature B cells prior to their differentiation into plasma cells. Alternative splicing results in two different isoforms, CD22α and CD22β. The CD22β monomer is the principally occurring isoform but CD22 also appears as a heterodimer of CD22β and the shorter isoform, CD22α. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | B cell receptor CD22 precursor antibody B lymphocyte cell adhesion molecule antibody B-cell receptor CD22 antibody B-lymphocyte cell adhesion molecule antibody BL CAM antibody BL-CAM antibody BLCAM antibody CD 22 antibody CD22 antibody CD22 antigen antibody CD22 molecule antibody CD22 protein antibody CD22_HUMAN antibody Lectin 2 antibody Leu14 antibody Lyb8 antibody MGC130020 antibody sialic acid binding Ig like lectin 2 antibody Sialic acid binding immunoglobulin like lectin 2 antibody Sialic acid-binding Ig-like lectin 2 antibody SIGLEC 2 antibody Siglec-2 antibody SIGLEC2 antibody T cell surface antigen Leu 14 antibody T-cell surface antigen Leu-14 antibody |
|---|---|
| Molecular Weight(MW) | 95 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P20273 |
|---|
Application
Fig1: Western blot analysis of CD22 on human CD22 recombinant protein using anti-CD22 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CD22 on HEK293 (1) and CD22-hIgGFc transfected HEK293 (2) cell lysate using anti-CD22 antibody at 1/1,000 dilution.
Application
ysis of CD22 on different cell lysate using anti-CD22 antibody at 1/1,000 dilution. Positive control： Line1: L1210 Line2: Hela Line3: HEK293 Line4: Jurkat Line5: OCM-1 Line6: A431 Line7: NIH/3T3
Application
Fig4: ICC staining CD22 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CD22 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-CD22 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with CD22 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CD22 recombinant protein, CD22-hIgGFc transfected HEK293 cell lysate, L1210, Hela, HEK293, Jurkat, OCM-1, A431, NIH/3T3 cell lysate, human cervical cancer tissue, human cerebellum tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
