Application
Fig1: Western blot analysis of ANAPC11 on human ANAPC11 recombinant protein using anti-ANAPC11 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ANAPC11 on HEK293 (1) and ANAPC11-hIgGFc transfected HEK293 (2) cell lysate using anti-ANAPC11 antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of Hela cells with ANAPC11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ANAPC11 [H6-F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANAPC 11 antibody ANAPC11 antibody Anaphase promoting complex subunit 11 (yeast APC11 homolog) antibody Anaphase promoting complex subunit 11 antibody Anaphase promoting complex subunit 11 homolog (yeast) antibody Anaphase promoting complex subunit 11 homolog antibody Anaphase-promoting complex subunit 11 antibody Apc 11 antibody Apc 11p antibody APC11 anaphase promoting complex subunit 11 homolog (yeast) antibody APC11 anaphase promoting complex subunit 11 homolog antibody APC11 antibody APC11_HUMAN antibody Apc11p antibody Cyclosome subunit 11 antibody Hepatocellular carcinoma associated RING finger protein antibody Hepatocellular carcinoma-associated RING finger protein antibody HSPC 214 antibody HSPC214 antibody MGC882 antibody Yeast APC 11 homolog antibody Yeast APC11 homolog antibody |
| Product description | Comprising more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to APC and activates APC's cyclin-ubiquitination activity. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC; thus preventing APC activation. APC11 is a RING-H2 finger protein that allows for the synthesis of multiubiquitin chains in the presence of Ubiquitin carrier protein 4 (Ubc4) and ubiquitin conjugating enzyme (E2). In addition, a heterodimeric complex of either Ubc4 or UbcH10 with APC11 and APC2 catalyzes the ubiquitination of human securin and cyclin B1. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ANAPC 11 antibody ANAPC11 antibody Anaphase promoting complex subunit 11 (yeast APC11 homolog) antibody Anaphase promoting complex subunit 11 antibody Anaphase promoting complex subunit 11 homolog (yeast) antibody Anaphase promoting complex subunit 11 homolog antibody Anaphase-promoting complex subunit 11 antibody Apc 11 antibody Apc 11p antibody APC11 anaphase promoting complex subunit 11 homolog (yeast) antibody APC11 anaphase promoting complex subunit 11 homolog antibody APC11 antibody APC11_HUMAN antibody Apc11p antibody Cyclosome subunit 11 antibody Hepatocellular carcinoma associated RING finger protein antibody Hepatocellular carcinoma-associated RING finger protein antibody HSPC 214 antibody HSPC214 antibody MGC882 antibody Yeast APC 11 homolog antibody Yeast APC11 homolog antibody |
|---|---|
| Molecular Weight(MW) | 10 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q9NYG5 |
|---|
Application
Fig1: Western blot analysis of ANAPC11 on human ANAPC11 recombinant protein using anti-ANAPC11 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ANAPC11 on HEK293 (1) and ANAPC11-hIgGFc transfected HEK293 (2) cell lysate using anti-ANAPC11 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with ANAPC11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ANAPC11 recombinant protein, ANAPC11-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
