Application
Fig1: Western blot analysis of ANAPC1 on human ANAPC1 recombinant protein using anti-ANAPC1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ANAPC1 on HEK293 (1) and ANAPC1-hIgGFc transfected HEK293 (2) cell lysate using anti-ANAPC1 antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of MCF-7 cells with ANAPC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ANAPC1 [C7-G9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANAPC 1 antibody Anapc1 antibody Anaphase promoting complex subunit 1 antibody anaphase-promoting complex 1 (meiotic checkpoint regulator) antibody Anaphase-promoting complex subunit 1 antibody Apc 1 antibody APC1 antibody APC1_HUMAN antibody Cyclosome subunit 1 antibody MCPR antibody Meiotic checkpoint regulator antibody Mitotic checkpoint regulator antibody Protein Tsg 24 antibody Protein Tsg24 antibody Testis-specific gene 24 protein antibody TSG 24 antibody TSG24 antibody |
| Product description | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. |
| Immunogen | Purified recombinant fragment of human ANAPC1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:250~1:1000 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ANAPC 1 antibody Anapc1 antibody Anaphase promoting complex subunit 1 antibody anaphase-promoting complex 1 (meiotic checkpoint regulator) antibody Anaphase-promoting complex subunit 1 antibody Apc 1 antibody APC1 antibody APC1_HUMAN antibody Cyclosome subunit 1 antibody MCPR antibody Meiotic checkpoint regulator antibody Mitotic checkpoint regulator antibody Protein Tsg 24 antibody Protein Tsg24 antibody Testis-specific gene 24 protein antibody TSG 24 antibody TSG24 antibody |
|---|---|
| Molecular Weight(MW) | 216 kDa |
| Cellular Localization | Anaphase-promoting complex, cytosol, nucleoplasm. |
| SwissProt ID | Q9H1A4 |
|---|
Application
Fig1: Western blot analysis of ANAPC1 on human ANAPC1 recombinant protein using anti-ANAPC1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ANAPC1 on HEK293 (1) and ANAPC1-hIgGFc transfected HEK293 (2) cell lysate using anti-ANAPC1 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of MCF-7 cells with ANAPC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ANAPC1 recombinant protein, ANAPC1-hIgGFc transfected HEK293 cell lysate, MCF-7. |
|---|---|
| Application Notes | WB:1:250~1:1000 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
