Application
Fig1: Western blot analysis of MRPL42 on human MRPL42 recombinant protein using anti-MRPL42 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of MRPL42 on HEK293 (1) and MRPL42-hIgGFc transfected HEK293 (2) cell lysate using anti-MRPL42 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of MRPL42 on different cell lysate using anti-MRPL42 antibody at 1/1,000 dilution. Positive control： Line1:HL7702 Line2:HepG2 Line3:SMMC-7721 Line4:HEK293 Line5:HeLa Line6:RajiApplication
Fig4: ICC staining MRPL42 (green) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-MRPL42 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-MRPL42 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HepG2 cells with MRPL42 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | MRPL42 [G11-H6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 28S ribosomal protein S32 antibody 39S ribosomal protein L31 antibody 39S ribosomal protein L42 antibody HSPC204 antibody L31mt antibody L42mt antibody mitochondrial antibody Mitochondrial ribosomal protein L42 antibody Mitochondrial ribosomal protein S32 antibody MRP L31 antibody MRP L42 antibody MRP S32 antibody MRP-L31 antibody MRP-L42 antibody MRP-S32 antibody MRPL31 antibody Mrpl42 antibody MRPS32 antibody PTD007 antibody RM42_HUMAN antibody RPML31 antibody S32mt antibody |
| Product description | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 28S ribosomal protein S32 antibody 39S ribosomal protein L31 antibody 39S ribosomal protein L42 antibody HSPC204 antibody L31mt antibody L42mt antibody mitochondrial antibody Mitochondrial ribosomal protein L42 antibody Mitochondrial ribosomal protein S32 antibody MRP L31 antibody MRP L42 antibody MRP S32 antibody MRP-L31 antibody MRP-L42 antibody MRP-S32 antibody MRPL31 antibody Mrpl42 antibody MRPS32 antibody PTD007 antibody RM42_HUMAN antibody RPML31 antibody S32mt antibody |
|---|---|
| Molecular Weight(MW) | 16 kDa |
| Cellular Localization | Mitochondrion. |
| SwissProt ID | Q9Y6G3 |
|---|
Application
Fig1: Western blot analysis of MRPL42 on human MRPL42 recombinant protein using anti-MRPL42 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of MRPL42 on HEK293 (1) and MRPL42-hIgGFc transfected HEK293 (2) cell lysate using anti-MRPL42 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of MRPL42 on different cell lysate using anti-MRPL42 antibody at 1/1,000 dilution. Positive control： Line1:HL7702 Line2:HepG2 Line3:SMMC-7721 Line4:HEK293 Line5:HeLa Line6:Raji
Application
Fig4: ICC staining MRPL42 (green) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-MRPL42 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-MRPL42 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HepG2 cells with MRPL42 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human MRPL42 recombinant protein, MRPL42-hIgGFc transfected HEK293 cell lysate, HL7702, HepG2, SMMC-7721, HEK293, HeLa, Raji cell lysate, HeLa, human esophageal cancer tissue, human cervical cancer tissue, HepG2. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
