Application
Fig1: Western blot analysis of WHSC2 on human WHSC2 recombinant protein using anti-WHSC2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of WHSC2 on HEK293 (1) and WHSC2-hIgGFc transfected HEK293 (2) cell lysate using anti-WHSC2 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of WHSC2 on different cell lysate using anti-WHSC2 antibody at 1/1,000 dilution. Positive control： Line1:Jurkat Line2:HeLa Line3:HEK293 Line4:Rat brain Line5:A549 Line6:SPC-A-1Application
Fig4: ICC staining WHSC2 (green) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HEK293 cells with WHSC2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | WHSC2 [H8-B11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ10442 antibody FLJ25112 antibody Negative elongation factor A antibody Negative elongation factor polypeptide A antibody NELF A antibody NELF-A antibody NELFA antibody NELFA_HUMAN antibody P/OKcl.15 antibody P/OKcl15 antibody WHSC 2 antibody Whsc2 antibody Wolf Hirschhorn syndrome candidate 2 antibody Wolf Hirschhorn syndrome candidate 2 protein antibody Wolf-Hirschhorn syndrome candidate 2 protein antibody |
| Product description | NELF-A, for negative elongation factor A, is a protein factor required for DRB-sensitive transcription. NELF-A is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b (P-TEFb) and negative transcription elongation factors, DSIF and NELF. DSIF and NELF act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. DSIF/NELF inhibition of transcription is prevented by P-TEFb in cooperation with FACT. NELF-A is also known as WHSC2 (Wolf-Hirschhorn syndrome candidate 2). Wolf-Hirschhorn syndrome is a multiple malformation syndrome characterized by mental and developmental defects resulting from a hemizygous deletion of the distal short arm of chromosome 4 (4p16.3). The human NELF-A gene maps to chromosome 4p16.3 and encodes a 528 amino acid protein that is expressed in endothelial cells. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | FLJ10442 antibody FLJ25112 antibody Negative elongation factor A antibody Negative elongation factor polypeptide A antibody NELF A antibody NELF-A antibody NELFA antibody NELFA_HUMAN antibody P/OKcl.15 antibody P/OKcl15 antibody WHSC 2 antibody Whsc2 antibody Wolf Hirschhorn syndrome candidate 2 antibody Wolf Hirschhorn syndrome candidate 2 protein antibody Wolf-Hirschhorn syndrome candidate 2 protein antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of WHSC2 on human WHSC2 recombinant protein using anti-WHSC2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of WHSC2 on HEK293 (1) and WHSC2-hIgGFc transfected HEK293 (2) cell lysate using anti-WHSC2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of WHSC2 on different cell lysate using anti-WHSC2 antibody at 1/1,000 dilution. Positive control： Line1:Jurkat Line2:HeLa Line3:HEK293 Line4:Rat brain Line5:A549 Line6:SPC-A-1
Application
Fig4: ICC staining WHSC2 (green) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HEK293 cells with WHSC2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human WHSC2 recombinant protein, WHSC2-hIgGFc transfected HEK293 cell lysate, Jurkat, HeLa, HEK293, A549, SPC-A-1 cell lysate, Rat brain tissue lysate, HeLa, human colon cancer tissue, human ovarian cancer tissue, HEK293. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
