Application
Fig1: Western blot analysis of PIWIL4 on human PIWIL4 recombinant protein using anti-PIWIL4 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PIWIL4 on HEK293 (1) and PIWIL4-hIgGFc transfected HEK293 (2) cell lysate using anti-PIWIL4 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of PIWIL4 on different cell lysate using anti-PIWIL4 antibody at 1/1,000 dilution. Positive control： Line1:PC-3 Line2:PANC-1Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PIWIL4 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PIWIL4 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with PIWIL4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PIWIL4 [B11-G9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686P01248 antibody FLJ36156 antibody HILI 2 antibody HILI2 antibody HIWI 2 antibody HIWI2 antibody Miwi 2 protein antibody Miwi2 antibody PIWI antibody Piwi like 2 antibody Piwi like 4 (Drosophila) antibody Piwi like 4 antibody Piwi like protein 4 antibody PIWI like protein antibody Piwi like RNA mediated gene silencing 4 antibody Piwi-like protein 4 antibody PIWIL 4 antibody Piwil4 antibody PIWL4_HUMAN antibody |
| Product description | PIWIL4 (PIWI-like protein 4), also known as HIWI2 is a 852 amino acid protein that belongs to the argonaute family. PIWIL4 contains one PAZ domain and one PIWI domain and is essential for the maintenance of germline stem cells. PIWIL4 is a cytoplasmic protein that is expressed in adult testis. It regulates spermatogenesis and primordial germ cell production and has an essential role in meiotic differentiation of spermatocytes and in self-renewal of spermatogonial stem cells. PIWIL4-null mice are of normal size and weight and have the expected life span. Homozygous PIWIL4-null females are fertile and have no obvious defects. However, PIWIL4-deficient males are infertile and show a meiotic progression defect in early prophase of meiosis I and progressive loss of germ cells with age. Mutant males show elevated expression of LINE-1 and intracisternal A particle (IAP) element transcripts in germ cell lineages. The gene encoding PIWIL4 maps to human chromosome 11. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | DKFZp686P01248 antibody FLJ36156 antibody HILI 2 antibody HILI2 antibody HIWI 2 antibody HIWI2 antibody Miwi 2 protein antibody Miwi2 antibody PIWI antibody Piwi like 2 antibody Piwi like 4 (Drosophila) antibody Piwi like 4 antibody Piwi like protein 4 antibody PIWI like protein antibody Piwi like RNA mediated gene silencing 4 antibody Piwi-like protein 4 antibody PIWIL 4 antibody Piwil4 antibody PIWL4_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 97 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
| SwissProt ID | Q7Z3Z4 |
|---|
Application
Fig1: Western blot analysis of PIWIL4 on human PIWIL4 recombinant protein using anti-PIWIL4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PIWIL4 on HEK293 (1) and PIWIL4-hIgGFc transfected HEK293 (2) cell lysate using anti-PIWIL4 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PIWIL4 on different cell lysate using anti-PIWIL4 antibody at 1/1,000 dilution. Positive control： Line1:PC-3 Line2:PANC-1
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PIWIL4 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PIWIL4 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with PIWIL4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PIWIL4 recombinant protein, PIWIL4-hIgGFc transfected HEK293 cell lysate, PC-3, PANC-1 cell lysate, human ovarian cancer tissue, human rectum cancer tissue, Hela. |
|---|---|
| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
