Application
Fig1: Western blot analysis of RPL18A on human RPL18A recombinant protein using anti-RPL18A antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RPL18A on different cell lysate using anti-RPL18A antibody at 1/1,000 dilution. Positive control： Line1:NIH3T3 Line2:HEK293 Line3:HL60 Line4:Jurka Line5:Raji Line6:MOLT4 Line7:HeLaApplication
Fig3: ICC staining RPL18A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RPL18A antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of HEK293 cells with RPL18A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | RPL18A [G10-G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2510019J09Rik antibody 60S ribosomal protein L18a antibody L18A antibody MGC72957 antibody Ribosomal protein L18A antibody Ribosomal protein L18a like protein antibody |
| Product description | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). This gene is co-transcribed with the U68 snoRNA, located within the third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | 2510019J09Rik antibody 60S ribosomal protein L18a antibody L18A antibody MGC72957 antibody Ribosomal protein L18A antibody Ribosomal protein L18a like protein antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Cytosolic large ribosomal subunit. |
Application
Fig1: Western blot analysis of RPL18A on human RPL18A recombinant protein using anti-RPL18A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RPL18A on different cell lysate using anti-RPL18A antibody at 1/1,000 dilution. Positive control： Line1:NIH3T3 Line2:HEK293 Line3:HL60 Line4:Jurka Line5:Raji Line6:MOLT4 Line7:HeLa
Application
Fig3: ICC staining RPL18A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RPL18A antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of HEK293 cells with RPL18A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human RPL18A recombinant protein, NIH3T3, HEK293, HL60, Jurka, Raji, MOLT4, HeLa cell lysate, HepG2, human kidney tissue, HEK293. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
