Application
Fig1: Western blot analysis of PDE1B on human PDE1B recombinant protein using anti-PDE1B antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PDE1B on HEK293 (1) and PDE1B-hIgGFc transfected HEK293 (2) cell lysate using anti-PDE1B antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of PDE1B on PC-12 cell lysate using anti-PDE1B antibody at 1/1,000 dilution.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PDE1B antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PDE1B antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of A549 cells with PDE1B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PDE1B [A3-C4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5''-cyclic nucleotide phosphodiesterase 1B antibody 63 kDa Cam PDE antibody 63 kDa Cam-PDE antibody Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B antibody Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase antibody Calcium/calmodulin-dependent 3'' antibody Calmodulin stimulated phosphodiesterase PDE1B1 antibody Cam PDE 1B antibody Cam PDE1B antibody Cam-PDE 1B antibody PDE 1B antibody PDE1B antibody PDE1B_HUMAN antibody PDE1B1 antibody PDES 1B antibody PDES1B antibody Phosphodiesterase 1B antibody Phosphodiesterase 1B calmodulin dependent antibody Presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B antibody |
| Product description | PDEs are key enzymes in signaling pathways that influence smooth muscle tone regulation. The PDE1 family are calmodulin-dependent (CaM-PDEs) that undergo stimulation through a calcium-calmodulin complex. Human PDE1B (PDE1B1) protein is present in neuronal cells of the cerebellum, hippocampus, and caudate, and lymphoblastoid lines, such as RPMI-8392 cells. PDE1B may participate in learning, memory, and regulation of phosphorylation of DARPP-32 in dopaminergic neurons. A splice variant known as PDE1B2 encodes a 516-amino acid protein and diverges from PDE1B1 by the replacement of the first 38 residues with an alternative 18 residues. The human PDE1B gene maps to chromosome 12q13, contains 13 exons, and encodes a 536 amino acid protein. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | 5''-cyclic nucleotide phosphodiesterase 1B antibody 63 kDa Cam PDE antibody 63 kDa Cam-PDE antibody Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B antibody Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase antibody Calcium/calmodulin-dependent 3'' antibody Calmodulin stimulated phosphodiesterase PDE1B1 antibody Cam PDE 1B antibody Cam PDE1B antibody Cam-PDE 1B antibody PDE 1B antibody PDE1B antibody PDE1B_HUMAN antibody PDE1B1 antibody PDES 1B antibody PDES1B antibody Phosphodiesterase 1B antibody Phosphodiesterase 1B calmodulin dependent antibody Presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of PDE1B on human PDE1B recombinant protein using anti-PDE1B antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PDE1B on HEK293 (1) and PDE1B-hIgGFc transfected HEK293 (2) cell lysate using anti-PDE1B antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PDE1B on PC-12 cell lysate using anti-PDE1B antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PDE1B antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PDE1B antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A549 cells with PDE1B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PDE1B recombinant protein, PDE1B-hIgGFc transfected HEK293 cell lysate, PC-12 cell lysate, human ovarian cancer tissue, human rectum cancer tissue, A549. |
|---|---|
| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
