Application
Fig1: Western blot analysis of DCTN4 on human DCTN4 recombinant protein using anti-DCTN4 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of DCTN4 on HEK293 (1) and DCTN4-hIgGFc transfected HEK293 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of DCTN4 on Raw264.7 (1) and NIH3T3 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.Application
Fig4: ICC staining DCTN4 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of A549 cells with DCTN4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | DCTN4 [D7-G9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCTN 4 antibody Dctn4 antibody DCTN4_HUMAN antibody Dynactin 4 (p62) antibody Dynactin 4 antibody Dynactin p62 subunit antibody Dynactin subunit 4 antibody Dynactin subunit p62 antibody Dynactin4 antibody p62 antibody |
| Product description | Dynactin is a multisubunit complex and a required cofactor for most, or all, of the cellular processes powered by the microtubule-based motor cytoplasmic dynein. Dynactin contains a short actin-related protein 1 (Arp1) filament with capZ at the barbed end and p62 at the pointed end. The p62 subunit is an integral component of 20 S dynactin with a highly conserved cysteine-rich motif that interacts directly with Arp1. Dynactin p62 has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin. In addition, Dynactin p62 is distributed in the nucleus at very high expression levels. Due to the structural composition of dynactin, the p62 subunit is implicated in Arp1 pointed-end binding and in linking dynein and dynactin to the cortical cytoskeleton. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | DCTN 4 antibody Dctn4 antibody DCTN4_HUMAN antibody Dynactin 4 (p62) antibody Dynactin 4 antibody Dynactin p62 subunit antibody Dynactin subunit 4 antibody Dynactin subunit p62 antibody Dynactin4 antibody p62 antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. |
| SwissProt ID | Q9UJW0 |
|---|
Application
Fig1: Western blot analysis of DCTN4 on human DCTN4 recombinant protein using anti-DCTN4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DCTN4 on HEK293 (1) and DCTN4-hIgGFc transfected HEK293 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of DCTN4 on Raw264.7 (1) and NIH3T3 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.
Application
Fig4: ICC staining DCTN4 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of A549 cells with DCTN4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human DCTN4 recombinant protein, DCTN4-hIgGFc transfected HEK293 cell lysate, Raw264.7, NIH3T3 cell lysate, HepG2, human cervical cancer tissue, human ovarian cancer tissue, A549. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
