Application
Fig1: Western blot analysis of ALCAM on human ALCAM recombinant protein using anti-ALCAM antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ALCAM on HEK293 (1) and ALCAM-hIgGFc transfected HEK293 (2) cell lysate using anti-ALCAM antibody at 1/1,000 dilution.Application
Fig3: ICC staining ALCAM (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-ALCAM antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-ALCAM antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Jurkat cells with ALCAM antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ALCAM [C7-E12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Activated leukocyte cell adhesion molecule (ALCAM), expressed on activated leukocytes T cells, B cells and monocytes, is a member of the immunoglobulin superfamily (IgSF) and identified as a CD6 ligand. CD6 is a type I membrane protein in the scavenger receptor cysteine rich protein superfamily that acts in T cell adhesion and costimulation. ALCAM mediates thymocyte-thymic epithelial cell adhesion via homophilic (ALCAM-ALCAM) and heterophilic (ALCAM-CD6) interactions involving a previously undescribed protein-protein interaction between a member of the scavenger receptor cysteine rich protein superfamily and the immunoglobulin superfamily. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCICCIHCWB |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Activated leukocyte cell adhesion molecule antibody ALCAM antibody ALCAM protein antibody CD 166 antibody CD166 antibody CD166 antigen antibody CD166_HUMAN antibody FLJ3851 antibody FLJ38514 antibody MEMD antibody MGC71733 antibody |
|---|---|
| Cellular Localization | Membrane. |
| SwissProt ID | Q13740 |
|---|
Application
Fig1: Western blot analysis of ALCAM on human ALCAM recombinant protein using anti-ALCAM antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ALCAM on HEK293 (1) and ALCAM-hIgGFc transfected HEK293 (2) cell lysate using anti-ALCAM antibody at 1/1,000 dilution.
Application
Fig3: ICC staining ALCAM (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-ALCAM antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-ALCAM antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Jurkat cells with ALCAM antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ALCAM recombinant protein, ALCAM-hIgGFc transfected HEK293 cell lysate, human cervical cancer tissue, human bladder cancer tissue, Hela, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
