Application
Fig1: Western blot analysis of CFLAR on human CFLAR recombinant protein using anti-CFLAR antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CFLAR on different cell lysate using anti-CFLAR antibody at 1/1,000 dilution. Positive control： Line1: Jurkat Line2: 3T3L1 Line3: RajiApplication
Fig3: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CFLAR antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-CFLAR antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Jurkat cells with CFLAR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CFLAR [D12-A3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | c FLIP antibody c FLIPL antibody c FLIPR antibody c FLIPS antibody c-FLIP antibody c-FLIPL antibody c-FLIPR antibody c-FLIPS antibody CASH antibody CASP8 and FADD like apoptosis regulator antibody CASP8 and FADD-like apoptosis regulator subunit p12 antibody CASP8AP1 antibody Caspase homolog antibody Caspase like apoptosis regulatory protein antibody Caspase related inducer of apoptosis antibody Caspase-eight-related protein antibody Caspase-like apoptosis regulatory protein antibody Casper antibody Cellular FLICE-like inhibitory protein antibody CFLA antibody Cflar antibody CFLAR_HUMAN antibody CLARP antibody FADD like anti apoptotic molecule antibody FADD-like antiapoptotic molecule 1 antibody FLAME antibody FLAME-1 antibody FLAME1 antibody FLIP antibody I FLICE antibody I-FLICE antibody Inhibitor of FLICE antibody MACH-related inducer of toxicity antibody MRIT antibody OTTHUMP00000163715 antibody OTTHUMP00000206475 antibody OTTHUMP00000206476 antibody OTTHUMP00000206478 antibody OTTHUMP00000206479 antibody OTTHUMP00000206480 antibody OTTHUMP00000206482 antibody OTTHUMP00000207360 antibody Usurpin antibody Usurpin beta antibody |
| Product description | FLIP (FLICE inhibitory protein) is expressed as both long and short forms and is involved in the regulation of apoptosis. The short form of FLIP contains two death effector domains homologous to the death effector domain of the FAS-associating protein FADD. The long form of FLIP, which shares significant homology with the cysteine protease FLICE, contains an additional caspase-like domain, but lacks a catalytic active site and lacks the residues that form the substrate binding pocket in most caspases. FLIP has been designated by independent groups as Casper, I-FLICE, CLARP, FLAME-1 and MRIT. Although its exact role is still being elucidated, FLIP appears to be an important factor in the regulation of apoptosis downstream of all known death receptors. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:100-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | c FLIP antibody c FLIPL antibody c FLIPR antibody c FLIPS antibody c-FLIP antibody c-FLIPL antibody c-FLIPR antibody c-FLIPS antibody CASH antibody CASP8 and FADD like apoptosis regulator antibody CASP8 and FADD-like apoptosis regulator subunit p12 antibody CASP8AP1 antibody Caspase homolog antibody Caspase like apoptosis regulatory protein antibody Caspase related inducer of apoptosis antibody Caspase-eight-related protein antibody Caspase-like apoptosis regulatory protein antibody Casper antibody Cellular FLICE-like inhibitory protein antibody CFLA antibody Cflar antibody CFLAR_HUMAN antibody CLARP antibody FADD like anti apoptotic molecule antibody FADD-like antiapoptotic molecule 1 antibody FLAME antibody FLAME-1 antibody FLAME1 antibody FLIP antibody I FLICE antibody I-FLICE antibody Inhibitor of FLICE antibody MACH-related inducer of toxicity antibody MRIT antibody OTTHUMP00000163715 antibody OTTHUMP00000206475 antibody OTTHUMP00000206476 antibody OTTHUMP00000206478 antibody OTTHUMP00000206479 antibody OTTHUMP00000206480 antibody OTTHUMP00000206482 antibody OTTHUMP00000207360 antibody Usurpin antibody Usurpin beta antibody |
|---|---|
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of CFLAR on human CFLAR recombinant protein using anti-CFLAR antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CFLAR on different cell lysate using anti-CFLAR antibody at 1/1,000 dilution. Positive control： Line1: Jurkat Line2: 3T3L1 Line3: Raji
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CFLAR antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-CFLAR antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Jurkat cells with CFLAR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CFLAR recombinant protein, CFLAR-hIgGFc transfected HEK293 cell lysate, Jurkat, 3T3L1, Raji cell lysate, human cervical cancer tissue, human esophagus cancer tissue, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:100-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
