Application
Fig1: Western blot analysis of SLC27A5 on human SLC27A5 recombinant protein using anti-SLC27A5 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of SLC27A5 on HEK293 (1) and SLC27A5-hIgGFc transfected HEK293 (2) cell lysate using anti-SLC27A5 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of SLC27A5 on 3T3L1 (1) and NIH3T3 (2) cell lysate using anti-SLC27A5 antibody at 1/1,000 dilution.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SLC27A5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-SLC27A5 antibody. Counter stained with hematoxylin.| Product Name | SLC27A5 [C10-B11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACSB antibody ACSVL6 antibody BA CoA ligase antibody BA-CoA ligase antibody BACS antibody BAL antibody Bile acid CoA ligase antibody Bile acid-CoA ligase antibody Bile acyl CoA synthetase antibody Bile acyl-CoA synthetase antibody Cholate CoA ligase antibody CholateCoA ligase antibody FACVL3 antibody FATP 5 antibody FATP-5 antibody FATP5 antibody Fatty acid Coenzyme A ligase very long chain 3 antibody Fatty acid transport protein 5 antibody Fatty-acid-coenzyme A ligase antibody FLJ22987 antibody S27A5_HUMAN antibody Slc27a5 antibody Solute carrier family 27 (fatty acid transporter) member 5 antibody Solute carrier family 27 member 5 antibody Very long chain acyl CoA synthetase homolog 2 antibody Very long chain acyl CoA synthetase related protein antibody very long-chain 3 antibody Very long-chain acyl-CoA synthetase homolog 2 antibody Very long-chain acyl-CoA synthetase-related protein antibody VLACS related antibody VLACS-related antibody VLACSR antibody VLCS H2 antibody VLCS-H2 antibody VLCSH2 antibody |
| Product description | Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:250~1:1000 IHC:1:20~1:100 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | ACSB antibody ACSVL6 antibody BA CoA ligase antibody BA-CoA ligase antibody BACS antibody BAL antibody Bile acid CoA ligase antibody Bile acid-CoA ligase antibody Bile acyl CoA synthetase antibody Bile acyl-CoA synthetase antibody Cholate CoA ligase antibody CholateCoA ligase antibody FACVL3 antibody FATP 5 antibody FATP-5 antibody FATP5 antibody Fatty acid Coenzyme A ligase very long chain 3 antibody Fatty acid transport protein 5 antibody Fatty-acid-coenzyme A ligase antibody FLJ22987 antibody S27A5_HUMAN antibody Slc27a5 antibody Solute carrier family 27 (fatty acid transporter) member 5 antibody Solute carrier family 27 member 5 antibody Very long chain acyl CoA synthetase homolog 2 antibody Very long chain acyl CoA synthetase related protein antibody very long-chain 3 antibody Very long-chain acyl-CoA synthetase homolog 2 antibody Very long-chain acyl-CoA synthetase-related protein antibody VLACS related antibody VLACS-related antibody VLACSR antibody VLCS H2 antibody VLCS-H2 antibody VLCSH2 antibody |
|---|---|
| Molecular Weight(MW) | 75 kDa |
| Cellular Localization | Endoplasmic reticulum membrane. |
| SwissProt ID | Q9Y2P5 |
|---|
Application
Fig1: Western blot analysis of SLC27A5 on human SLC27A5 recombinant protein using anti-SLC27A5 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of SLC27A5 on HEK293 (1) and SLC27A5-hIgGFc transfected HEK293 (2) cell lysate using anti-SLC27A5 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of SLC27A5 on 3T3L1 (1) and NIH3T3 (2) cell lysate using anti-SLC27A5 antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SLC27A5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-SLC27A5 antibody. Counter stained with hematoxylin.| Positive Control | Human SLC27A5 recombinant protein, SLC27A5-hIgGFc transfected HEK293 cell lysate, 3T3L1, NIH3T3 cell lysate, human liver cancer tissue, human stomach tissue. |
|---|---|
| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
