Application
Fig1: Western blot analysis of PSAP on human PSAP recombinant protein using anti-PSAP antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PSAP on HEK293 (1) and PSAP-hIgGFc transfected HEK293 (2) cell lysate using anti-PSAP antibody at 1/1,000 dilution.Application
Fig3: ICC staining PSAP (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PSAP antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with PSAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | PSAP [F4-D5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A1 activator antibody Cerebroside sulfate activator antibody Co-beta-glucosidase antibody Component C antibody CSAct antibody Dispersin antibody GLBA antibody Glucosylceramidase activator antibody Proactivator polypeptide antibody Proactivator polypeptide precursor antibody Prosaposin (sphingolipid activator protein 1) antibody prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) antibody Prosaposin antibody Protein A antibody Protein C antibody PSAP antibody SAP-1 antibody SAP-2 antibody SAP_HUMAN antibody SAP1 antibody Saposin A antibody Saposin B antibody Saposin B Val antibody Saposin C antibody Saposin D antibody Saposin-D antibody Saposins antibody Sgp1 antibody Sphingolipid activator protein 1 antibody Sphingolipid activator protein 2 antibody Sulfated glycoprotein 1 antibody Sulfatide/GM1 activator antibody |
| Product description | Puromycin-sensitive aminopeptidase (PSA or PSAP) is a 100kDa zinc metallopeptidase which degrades neuropeptides by removing amino acid residues from the amino-terminus. The protein is the most abundant aminopeptidase in the brain, however it is not exclusive to that organ. It is localized primarily in the cytoplasm, and plays a role in the metabolism of neuropeptides in nerve terminals and synaptic clefts. The human PSA gene maps to chromosome 17q 2-32. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | A1 activator antibody Cerebroside sulfate activator antibody Co-beta-glucosidase antibody Component C antibody CSAct antibody Dispersin antibody GLBA antibody Glucosylceramidase activator antibody Proactivator polypeptide antibody Proactivator polypeptide precursor antibody Prosaposin (sphingolipid activator protein 1) antibody prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) antibody Prosaposin antibody Protein A antibody Protein C antibody PSAP antibody SAP-1 antibody SAP-2 antibody SAP_HUMAN antibody SAP1 antibody Saposin A antibody Saposin B antibody Saposin B Val antibody Saposin C antibody Saposin D antibody Saposin-D antibody Saposins antibody Sgp1 antibody Sphingolipid activator protein 1 antibody Sphingolipid activator protein 2 antibody Sulfated glycoprotein 1 antibody Sulfatide/GM1 activator antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Lysosome. |
| SwissProt ID | P07602 |
|---|
Application
Fig1: Western blot analysis of PSAP on human PSAP recombinant protein using anti-PSAP antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PSAP on HEK293 (1) and PSAP-hIgGFc transfected HEK293 (2) cell lysate using anti-PSAP antibody at 1/1,000 dilution.
Application
Fig3: ICC staining PSAP (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PSAP antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with PSAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | Human PSAP recombinant protein, PSAP-hIgGFc transfected HEK293 cell lysate, HepG2. human pancreas tissue, Hela. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
