Application
Fig1: Western blot analysis of NME1 on NME1-hIgGFc transfected HEK293 (2) cell lysate using anti-NME1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining NME1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NME1 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of Jurkat cells with NME1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Product Name | NME1 [C6-B2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AWD antibody AWD, drosophila, homolog of antibody GAAD antibody Granzyme A activated DNase antibody Granzyme A-activated DNase antibody GZMA activated DNase antibody Metastasis inhibition factor NM23 antibody NB antibody NBS antibody NDK A antibody NDKA antibody NDKA_HUMAN antibody NDP kinase A antibody NDPK-A antibody NDPKA antibody NM23 antibody NM23 long variant, included antibody nm23-H1 antibody NM23-M1 antibody NM23H1B, included antibody NME/NM23 nucleoside diphosphate kinase 1 antibody Nme1 antibody NME1-NME2 spliced read-through transcript, included antibody Non-metastatic cells 1, protein (NM23A) expressed in antibody Nonmetastatic cells 1, protein expressed in antibody Nonmetastatic protein 23 antibody Nonmetastatic protein 23, homolog 1 antibody Nucleoside diphosphate kinase A antibody Tumor metastatic process-associated protein antibody |
| Product description | The nm23 protein is implicated in a variety of biological processes, including suppressing metastasis, phytochrome responses in plants and regulating differentiation. Nm23-H1, nm23-H2 and nm23-H2, members of the nm23 family, are putative metastasis suppressor genes that encode nucleoside diphosphate kinase (NDPK) A and B. NDPKs form oligomers, which are distributed in both the soluble and particulate fractions of cells. Upon cAMP-induced differentiation of rat cells, nm23 proteins show a differential interaction with intermediate filaments. Both isoforms also associate with GFAP in differentiated cells. nm23 proteins and their mutants are localized predominantly in the mitochondria. Nm23 mutants are unable to inhibit differentiation and promote apoptosis as a results of defective protein-protein interactions in the mitochondria. Nm23-H1, nm23-H2 and nm23-H3 are indicators of a poor prognosis in human hematopoietic malignancies, and as a high expression of nm23-H1 and -H2 is positively correlated with histological |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AWD antibody
AWD drosophila homolog of antibody GAAD antibody Granzyme A activated DNase antibody Granzyme A-activated DNase antibody GZMA activated DNase antibody Metastasis inhibition factor NM23 antibody NB antibody NBS antibody NDK A antibody NDKA antibody NDKA_HUMAN antibody NDP kinase A antibody NDPK-A antibody NDPKA antibody NM23 antibody NM23 long variant included antibody nm23-H1 antibody NM23-M1 antibody NM23H1B included antibody NME/NM23 nucleoside diphosphate kinase 1 antibody Nme1 antibody NME1-NME2 spliced read-through transcript included antibody Non-metastatic cells 1 protein (NM23A) expressed in antibody Nonmetastatic cells 1 protein expressed in antibody Nonmetastatic protein 23 antibody Nonmetastatic protein 23 homolog 1 antibody Nucleoside diphosphate kinase A antibody Tumor metastatic process-associated protein antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | P15531 |
|---|
Application
Fig1: Western blot analysis of NME1 on NME1-hIgGFc transfected HEK293 (2) cell lysate using anti-NME1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining NME1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NME1 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Jurkat cells with NME1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | NME1-hIgGFc transfected HEK293 cell lysate, human placenta tissue, Hela, Jurkat. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
